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HelpTest ID 60483 Human Papillomavirus (HPV), High-Risk, DNA In Situ Hybridization
Useful For
Detection of human papillomavirus DNA from high-risk genotypes (16, 18, 31, 33 and 51)
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| 62886 | HPV High Risk E6/E7, RNA ISH | No | No |
Testing Algorithm
HPV high risk E6/E7 will be performed at the discretion of the Mayo pathologist.
Method Name
In Situ Hybridization
Reporting Name
HPV High Risk, DNA ISHSpecimen Type
TissueSpecimen Type: Formalin-fixed, paraffin-embedded tissue block; or 5 unstained glass, positively charged slides with 5 (+ or - 1) microns formalin-fixed, paraffin-embedded tissue
Container/Tube: Pathology Packaging Kit (Supply T554)
Collection Instructions: Attach the green pathology address label included in the kit to the outside of the transport container.
Additional Information:
1. A pathology/diagnostic report and a brief history are required.
2. One slide will be stained with hematoxylin and eosin and retained.
Forms: If not ordering electronically, complete, print, and send an Oncology Test Request Form (T729) with the specimen
(http://www.mayomedicallaboratories.com/it-mmfiles/oncology-request-form.pdf)
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Tissue | Ambient (preferred) | |
| Refrigerated | ||
Clinical Information
Persistent infections with high risk human papillomavirus (HPV) genotypes (16, 18, 31, 33 and 51) are associated with cervical, vaginal, vulvar, and head and neck malignancies. Patients with HPV-related oropharyngeal squamous cell carcinoma (OPSCC) have shown better disease-specific survival and overall survival when compared to HPV-negative cases of OPSCC.
Cautions
The probe set used in this human papillomavirus (HPV) DNA in situ hybridization (ISH) test cannot detect all potential HPV serotypes that are associated with oropharyngeal squamous cell carcinoma. Following a negative HPV High Risk DNA ISH result, a more sensitive test, High Risk HPV RNA ISH, could be performed, if clinically indicated. The RNA ISH test is only available in the context of a pathology consultation (test 70012 / Pathology Consultation).
Day(s) Performed
Monday through Friday
Report Available
5 daysPerforming Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
88365-Primary
88364-If additional probe stain, in situ