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Test ID 62886 Human Papillomavirus (HPV) High Risk E6/E7, RNA In Situ Hybridization

Useful For

Stratification of  oropharyngeal squamous cell carcinoma

Testing Algorithm

This is not an orderable test. Order 80172 / Human Papillomavirus (HPV) Typing, DNA In Situ Hybridization or 60483 / Human Papillomavirus (HPV), High-Risk, DNA In Situ Hybridization. The Mayo pathologist will determine if this test is clinically indicated.

Method Name

In Situ Hybridization

Reporting Name

HPV High Risk E6/E7, RNA ISH

Specimen Type

Tissue

This is not an orderable test. Order 80172 / Human Papillomavirus (HPV) Typing, DNA In Situ Hybridization or 60483 / Human Papillomavirus (HPV), High-Risk, DNA In Situ Hybridization. The Mayo pathologist will determine if this test is clinically indicated.

Specimen Stability Information

Specimen Type Temperature Time
Tissue Ambient (preferred)
  Refrigerated 

Clinical Information

This assay is intended to identify the presence of human papillomavirus (HPV) E6/E7 transcripts from high-risk genotypes. This test has been shown to be more sensitive than HPV DNA in situ hybridization (ISH). Patients with HPV-related oropharyngeal squamous cell carcinoma (OPSCC) have shown better disease-specific survival and overall survival when compared to HPV-negative cases of OPSCC. An indication for this test is p16 expression by immunohistochemistry and negative HPV DNA ISH. In this instance, consideration should be given to the possibility of a false-negative HPV DNA ISH result.

Reference Values

Results are reported as positive or negative for types 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82. 

Cautions

No significant cautionary statements

Day(s) Performed

Monday through Friday

Report Available

5 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88365-Primary

88364-GC, if additional probe stain, in situ

NY State Approved

Yes