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HelpTest ID 64559 Neurofilament (SMI31), Immunostain Without Interpretation
Useful For
Differentiating neurons (neurofilament positive) from glia (neurofilament negative)
Method Name
Immunohistochemistry
Reporting Name
Neurofilament (SMI31) IHC, no interpSpecimen Type
TissueSpecimen Type: Tissue
Container/Tube: Immunostain Technical Only Envelope (T693)
Preferred: Formalin-fixed, paraffin-embedded (FFPE) tissue block
Acceptable: 2 unstained positively charged glass slides (25 x 75 x 1 mm) per test ordered; sections 4 microns thick.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Tissue | Ambient (preferred) | |
| Refrigerated | ||
Clinical Information
Neurofilament antibody clone SMI 31 reacts with a phosphorylated epitope on neurofilament H and, to a lesser degree, neurofilament M. Both of these proteins contain multiple tandemly repeated serine phosphorylation sites. Clone SMI 31 reacts with thick and thin axons and specific dendrites such as basket cell dendrites. SMI 31 may also stain neuronal cell bodies in pathological conditions.
Cautions
No significant cautionary statements.
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
88342-TC, primary
88341-TC, if addt'l IHC