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HelpTest ID 80172 Human Papillomavirus (HPV) Typing, DNA In Situ Hybridization
Useful For
Detection of both low-risk (6, 11) and high-risk genotypes (16, 18, 31, 33, and 51) of human papillomavirus DNA in paraffin-embedded human tissue
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| 62886 | HPV High Risk E6/E7, RNA ISH | No | No |
Testing Algorithm
HPV high risk E6/E7 will be performed at the discretion of the Mayo pathologist.
Method Name
In Situ Hybridization
Reporting Name
Human Papillomavirus (HPV) Typing, DNA ISHSpecimen Type
TissueSpecimen Type: Formalin-fixed, paraffin-embedded tissue block; or 6 unstained glass, positively charged slides with 5 (+ or - 1) microns formalin-fixed, paraffin-embedded tissue
Container/Tube: Pathology Packaging Kit (Supply T554)
Collection Instructions: Attach the green pathology address label included in the kit to the outside of the transport container.
Additional Information:
1. A pathology/diagnostic report and a brief history are required.
2. One slide will be stained with hematoxylin and eosin and retained.
Forms: If not ordering electronically, complete, print, and send an Oncology Test Request Form (T729) with the specimen
(http://www.mayomedicallaboratories.com/it-mmfiles/oncology-request-form.pdf)
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Tissue | Ambient (preferred) | |
| Refrigerated | ||
Clinical Information
Human papillomavirus (HPV) infections with low-risk genotypes (6, 11) can cause benign hyperplasia such as condylomas and papillomas. Persistent infections with high-risk genotypes (16, 18, 31, 33, and 51) are associated with cervical, vaginal, vulvar, and head and neck malignancies. Patients with HPV-related oropharyngeal squamous cell carcinoma (OPSCC) have shown better disease-specific survival and overall survival when compared to HPV-negative cases of OPSCC.
Reference Values
Results are reported as positive or negative for low-risk genotypes 6, 11, and/or high-risk genotypes 16, 18, 31, 33, and 51.
If additional interpretation/analysis is needed, request 70012 / Pathology Consultation along with this test.
Cautions
The probe set used in this human papillomavirus (HPV) DNA in situ hybridization (ISH) test cannot detect all potential HPV serotypes that are associated with oropharyngeal squamous cell carcinoma. Following a negative DNA ISH result, a more sensitive in situ RNA test may be performed, if clinically indicated, at the discretion of the Mayo pathologist.
Day(s) Performed
Monday through Friday
Report Available
5 daysPerforming Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
88365-Primary
88364-If additional probe stain, in situ