Home
HelpTest ID 81107 JC Virus (JCV) Detection by In Situ Hybridization
Useful For
Confirming a clinical and histopathologic diagnosis of progressive multifocal leukoencephalopathy (PML)
Method Name
In Situ Hybridization
Reporting Name
JC Virus by In Situ HybridizationSpecimen Type
TissueSpecimen Type: Formalin-fixed, paraffin-embedded tissue block; or 4 unstained glass, "positively charged" slides with 5 (+ or - 1) microns formalin-fixed, paraffin-embedded tissue
Container/Tube: Pathology Packaging Kit (Supply T554)
Collection Instructions: Attach the green pathology address label included in the kit to the outside of the transport container.
Additional Information:
1. A pathology/diagnostic report and a brief history are required.
2. One slide will be stained with hematoxylin and eosin and retained.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Tissue | Ambient (preferred) | |
| Refrigerated | ||
Clinical Information
JC virus (JCV) is the etiologic agent of progressive multifocal leukoencephalopathy (PML), a rare, demyelinating, fatal disorder of the central nervous system which occurs on a background of immune deficiency. PML is an infrequent complication of a wide variety of conditions, including lymphoproliferative disorders (Hodgkin disease, chronic lymphocytic leukemia), sarcoidosis, tuberculosis, and immunodeficiency diseases (AIDS).
Cautions
Studies have shown potential cross reactivity between BK and JCV ISH testing (Fritzsche FR, Pianca S, Gaspert A, et al: Silver-enhanced in situ hybridization for detection of polyomavirus DNA in patients with BK virus nephropathy. Diagn Mol Pathol. 2011 20(2):105-10).
Day(s) Performed
Monday through Friday; 9 a.m.
Report Available
5 daysPerforming Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
88365-Primary
88364-If additional probe stain, in situ