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Test ID AHEP Acute Viral Hepatitis Profile, Serum

Method Name

HAIGM, HBAG, HBIM, HCVDX, HBGNT: Electrochemiluminescence Immunoassay (ECLIA)

HCVQN: Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR)


Necessary Information


Date of collection is required.



Specimen Required


Patient Preparation: For 24 hours before specimen collection, patient should not take multivitamins or dietary supplements (eg, hair, skin, and nail supplements) containing biotin (vitamin B7).

Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container/Tube: Serum gel (red-top tubes are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 2.7 mL

Collection Instructions:

1. Centrifuge blood collection tube per manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).

2. Aliquot 2 mL serum into a plastic vial labeled as SST Serum, and ship frozen (preferred).


Reporting Name

Acute Hepatitis Profile

Specimen Type

Serum SST

Specimen Stability Information

Specimen Type Temperature Time
Serum SST Frozen (preferred) 84 days
  Refrigerated  6 days

Report Available

Same day/1 to 2 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

LOINC Code Information

Test ID Test Order Name Order LOINC Value
AHEP Acute Hepatitis Profile 24363-4

 

Result ID Test Result Name Result LOINC Value
HCVA4 HCV Ab, S 40726-2
HBIM HBc IgM Ab, S 24113-3
H_BAG HBs Antigen, S 5196-1
HAIGM Hepatitis A IgM Ab, S 13950-1

Forms

If not ordering electronically, complete, print, and send 1 of the following:

-Gastroenterology and Hepatology Test Request (T728)

-Infectious Disease Serology Test Request (T916)

Useful For

Differential diagnosis of recent acute viral hepatitis

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
HCVQN HCV RNA Detect/Quant, S Yes No
HBGNT HBs Antigen Confirmation, S Yes No

Testing Algorithm

If the hepatitis C virus (HCV) antibody result is reactive, then HCV RNA detection and quantification by real-time reverse transcription-polymerase chain reaction will be performed at an additional charge.

 

If the hepatitis B surface antigen result is reactive, then confirmation will be performed at an additional charge.

 

The following algorithms are available:

-Hepatitis B: Testing Algorithm for Screening, Diagnosis, and Management

-Hepatitis C: Testing Algorithm for Screening and Diagnosis

-Viral Hepatitis Serologic Profiles

Day(s) Performed

Monday through Saturday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

80074 (if all 4 initial tests are performed)

86709 (if all 4 are not performed)

86705 (if all 4 are not performed)

87340 (if all 4 are not performed)

86803 (if all 4 are not performed)

87522 (if appropriate)

87341 (if appropriate)

Profile Information

Test ID Reporting Name Available Separately Always Performed
HAIGM Hepatitis A IgM Ab, S Yes Yes
HBAG HBs Antigen, S Yes Yes
HBIM HBc IgM Ab, S Yes Yes
HCVDX HCV Ab w/Reflex to HCV PCR, S Yes Yes

Specimen Minimum Volume

1.9 mL

Reference Values

HEPATITIS B SURFACE ANTIGEN

Negative

 

HEPATITIS B SURFACE ANTIGEN CONFIRMATION

Negative

 

HEPATITIS B CORE IgM ANTIBODY

Negative

 

HEPATITIS A IgM ANTIBODY

Negative

 

HEPATITIS C ANTIBODY

Negative

 

HEPATITIS C VIRUS RNA DETECTION AND QUANTIFICATION BY REAL-TIME RT-PCR

Undetected