Home
HelpTest ID B6PRO Vitamin B6 Profile (PLP and PA), Plasma
Useful For
Determining the overall success of a vitamin B6 supplementation program
Diagnosis and evaluation of hypophosphatasia
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| PLP | Pyridoxal 5-Phosphate (PLP), P | Yes | Yes |
| B6PA | Pyridoxic Acid (PA), P | No | Yes |
Method Name
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Vitamin B6 Profile (PLP and PA), PSpecimen Type
Plasma HeparinCollection Container/Tube: Green top (sodium or lithium heparin)
Submission Container/Tube: Amber vial (T192)
Specimen Volume: 1 mL
Collection Instructions:
1. Fasting-overnight (12-14 hour) (infants-draw prior to next feeding).
2. Patient must not ingest vitamin supplements for 24 hours before the specimen is drawn.
3. Centrifuge at 4° C, then aliquot all plasma into amber vial to protect from light.
Forms: If not ordering electronically, complete, print, and send a Neurology Test Request Form-General (T732) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/neurology-request-form.pdf)
Specimen Minimum Volume
0.75 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma Heparin | Refrigerated (preferred) | 7 days |
| Frozen | 14 days |
Clinical Information
Vitamin B6 is a complex of 6 vitamers: pyridoxal, pyridoxol, pyridoxamine, and their 5'-phosphate esters. Due to its role as a cofactor in a number of enzymatic reactions, pyridoxal phosphate (PLP) has been determined to be the biologically active form of vitamin B6.
Vitamin B6 deficiency is a potential cause of burning mouth syndrome and a possible potentiating factor for carpal tunnel and tarsal tunnel syndromes. Persons who present chronic, progressive nerve compression disorders may be deficient in vitamin B6 and should be evaluated. Vitamin B6 deficiency is associated with symptoms of scaling of the skin, severe gingivitis, irritability, weakness, depression, dizziness, peripheral neuropathy, and seizures. In the pediatric population, deficiencies have been characterized by diarrhea, anemia, and seizures.
Markedly elevated PLP in conjunction with low levels of pyridoxic acid (PA) are observed in cases of hypophosphatasia, a disorder characterized by low levels of alkaline phosphatase and a range of skeletal abnormalities.
Reference Values
PYRIDOXAL 5-PHOSPHATE
5-50 mcg/L
PYRIDOXIC ACID
3-30 mcg/L
Cautions
Reference ranges were established using healthy fasting volunteers who abstained from vitamin supplementation for 24 hour prior to draw. Vitamin supplementation and nonfasting may result in elevated plasma vitamin concentrations.
Day(s) Performed
Two times per week; Days vary
Performing Laboratory
Mayo Medical Laboratories in New England
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
82542-Quantitative
84207-Pyridoxal phosphate (vitamin B6)