Test ID BARBX Barbiturates Confirmation, Chain of Custody, Urine
Useful For
Detecting drug abuse involving barbiturates such as amobarbital, butalbital, pentobarbital, phenobarbital, and secobarbital
Chain-of-custody is required whenever the results of testing could be used in a court of law. Its purpose is to protect the rights of the individual contributing the specimen by demonstrating that it was under the control of personnel involved with testing the specimen at all times; this control implies that the opportunity for specimen tampering would be limited.
Additional Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
COCH | Chain of Custody Processing | No | Yes |
ADLTX | Adulterants Survey, CoC, U | Yes | Yes |
Testing Algorithm
Adulterants testing will be performed on all chain of custody urine samples as per regulatory requirements.
Method Name
Immunoassay/Gas Chromatography-Mass Spectrometry (GC-MS) Confirmation with Quantitation
Reporting Name
Barbiturates Confirmation, CoC, USpecimen Type
UrineContainer/Tube: Chain-of-Custody Kit (T282) containing the specimen containers, seals, and documentation required.
Specimen Volume: 20 mL
Collection Instructions: Collect specimen in the container provided, seal, and submit with the associated documentation to satisfy the legal requirements for chain-of-custody testing.
Additional Information: Submitting <20 mL will compromise our ability to perform all necessary testing.
Forms: Chain-of-Custody Request Form is included in the Chain-of-Custody Kit (T282). A copy of this form is also available at http://www.mayomedicallaboratories.com/it-mmfiles/chain-of-custody-request-form.pdf.
Specimen Minimum Volume
5 mL
Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Urine | Refrigerated (preferred) | 14 days |
Frozen | 14 days | |
Ambient | 72 hours |
Clinical Information
Barbiturates represent a class of drugs that were originally introduced as sleep inducers. Butalbital is also used to control severe headaches. Mephobarbital and phenobarbital are frequently used to control major motor (grand mal) seizures. These drugs are commonly abused as "downers" to induce sleep after an amphetamine- or cocaine-induced "high."
Chain-of-custody is a record of the disposition of a specimen to document who collected it, who handled it, and who performed the analysis. When a specimen is submitted in this manner, analysis will be performed in such a way that it will withstand regular court scrutiny.
Reference Values
Negative
Cutoff concentrations:
IMMUNOASSAY SCREEN
<200 ng/mL
BUTALBITAL BY GC-MS
<100 ng/mL
AMOBARBITAL BY GC-MS
<100 ng/mL
PENTOBARBITAL BY GC-MS
<100 ng/mL
SECOBARBITAL BY GC-MS
<100 ng/mL
PHENOBARBITAL BY GC-MS
<100 ng/mL
Cautions
No significant cautionary statements. This test is very specific for the compounds tested.
Day(s) Performed
Monday through Friday
Report Available
2 daysPerforming Laboratory

Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
80345
G0480 (if appropriate)