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Test ID BBLD Bacterial Culture, Blood

Useful For

Diagnosis and treatment of the etiologic agent of sepsis

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
COMM Identification Commercial Kit No No
RMALD Ident by MALDI-TOF mass spec No No
GID Bacteria Identification No No
ISAE Aerobe Ident by Sequencing No No
REFID Additional Identification Procedure No No
SALS Serologic Agglut Method 1 Ident No No
EC Serologic Agglut Method 2 Ident No No
SHIG Serologic Agglut Method 3 Ident No No
STAP Identification Staphylococcus No No
STRP Identification Streptococcus No No
BLA Beta Lactamase No No
KPND1 KPC and NDM PCR No, Internal (Bill Only); Yes External (Order KPNRP) No
HODGB Carbapenamase-Modified Hodge Test No, Internal (Bill Only); Yes External (Order HODGE) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed and charged.

Method Name

Automated or Manual Detection of Positive Blood Cultures with Conventional Identification Methods

Reporting Name

Bacterial Culture, Blood

Specimen Type

Whole Blood Culture

This test is available by special request only-preapproval by testing lab is required.

 

Collection Instructions: Incubate blood bottles at 35° C for 24 hours before sending.

Additional Information:

1. It is strongly recommend that blood bottles are incubated at client location on an automated instrument until they flag positive and Gram stain confirms growth is present. The bottles can then be submitted under test IDENT / Organism Referred for Identification, Aerobic Bacteria.

2. Include a completed microbiology request form.

Forms: If not ordering electronically, complete, print, and send a Neurology Test Request Form-General (T732) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/neurology-request-form.pdf)

Specimen Minimum Volume

10-20 mL for adults; 1-3 mL for pediatric patients

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood Culture Ambient

Clinical Information

Bacteremia results when bacteria multiply at a rate that exceeds removal by phagocytes. The clinical pattern of bacteremia may be transient, intermittent, or continuous. Transient bacteremia often occurs after manipulative procedures (dental procedures, cystoscopy) or surgery in contaminated areas of the body. Undrained abscesses (intraabdominal, pelvic, hepatic) may result in intermittent bacteremia. A hallmark feature of subacute bacterial endocarditis is a continuous bacteremia.

 

The sources of bacteremia are genitourinary tract (25%), respiratory tract (20%), abscesses (10%), surgical wounds (5%), biliary tract (5%), other known sites (10%), and unknown sites (25%).

Reference Values

No growth

Identification of all organisms

Cautions

As a general guideline, 2 sets of cultures should be collected per febrile episode in adult patients, no more than 4 blood culture sets should be collected in a 24-hour period, and blood cultures should be collected prior to administration of antibiotics.

Day(s) Performed

Monday through Sunday; Continuously

Report Available

5 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87040-Bacterial Culture, Blood

87077-Identification Commercial Kit (if appropriate)

87077-Ident by MALDI-TOF Mass Spec (if appropriate)

87076-Id MALDI-TOF Mass Spec Anaerobe (if appropriate)

87077-Bacteria Identification (if appropriate)

87153-Aerobe Ident by Sequencing (if appropriate)

87153-Anaerobe Ident by Sequencing (if appropriate)

87077-Additional Identification Procedure (if appropriate)

87147 x 3-Serologic Agglut Method 1 Ident (if appropriate)

87147-Serologic Agglut Method 2 Ident (if appropriate)

87147 x 4-Serologic Agglut Method 3 Ident (if appropriate)

87077-Identification Staphylococcus (if appropriate)

87077-Identification Streptococcus (if appropriate)

87185-Beta Lactamase (if appropriate)

87798 x 2-KPC and NDM PCR (if appropriate)

87185-Carbapenemase Detection, Modified Hodge Test (if appropriate)

NY State Approved

Yes