Home
HelpTest ID BLPF B-Cell Lymphoma, FISH, Blood or Bone Marrow
Useful For
Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with various B-cell lymphomas
Tracking known chromosome abnormalities and response to therapy in patients with B-cell neoplasms
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| _PBCT | Probe, +2 | No, (Bill Only) | No |
| _PADD | Probe, +1 | No, (Bill Only) | No |
| _PB02 | Probe, +2 | No, (Bill Only) | No |
| _PB03 | Probe, +3 | No, (Bill Only) | No |
| _IL25 | Interphases, <25 | No, (Bill Only) | No |
| _I099 | Interphases, 25-99 | No, (Bill Only) | No |
| _I300 | Interphases, ≥100 | No, (Bill Only) | No |
Testing Algorithm
This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.
See Malignant Lymphoma, Guideline for Bone Marrow Staging Studies in Special Instructions.
Depending on the lymphoma subtype suspected, the most appropriate probes to order are listed in the table "Common Chromosome Abnormalities in B-cell Lymphomas" in Clinical Information.
If the patient is being tracked for known abnormalities, indicate which probes should be used.
If no probes (or lymphoma subtype) are specified at the time of order, a panel of the following probe sets will be performed:
-8q24.1 rearrangement, MYC
-18q21 rearrangement,
BCL2
-3q27 rearrangement, BCL6
-t(11;14),
CCND1/IGH
-17p-, TP53/D17Z1
-18q21 rearrangement,
MALT1
This assay detects chromosome abnormalities observed in the blood and bone marrow of patients with B-cell lymphoma. For testing paraffin-embedded tissue samples from patients with B-cell lymphoma, see BLYMF / B-Cell Lymphoma, FISH, Tissue.
Special Instructions
Method Name
Fluorescence In Situ Hybridization (FISH)
Reporting Name
B-cell Lymphoma, FISH, B/BMSpecimen Type
VariesProvide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
Forms: If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request Form (T726) with the specimen
(http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf)
Advise Express Mail or equivalent if not on courier service.
Submit only 1 of the following specimens:
Specimen Type: Blood
Container/Tube: Green top (sodium heparin)
Specimen Volume: 7-10 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Other anticoagulants are not recommended and are harmful to the viability of the cells.
Specimen Type: Bone marrow
Container/Tube: Green top (sodium heparin)
Specimen Volume: 1-2 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Other anticoagulants are not recommended and are harmful to the viability of the cells.
Acceptable:
Specimen Type: Touch prep or fresh tissue
Specimen Minimum Volume
Blood: 2 mL; Bone Marrow: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Clinical Information
Lymphoid neoplasms are known to be complex and the prognosis and clinical course of patients with lymphoma is highly variable. Genetic abnormalities have emerged as one of the most reliable criteria for categorizing lymphomas. Several chromosome abnormalities and variants of these abnormalities have been associated with various kinds of lymphoma (see Table).
|
Common Chromosome Abnormalities in B-cell Lymphomas |
||
|
Lymphoma Type |
Chromosome Abnormality |
FISH Probe |
|
Burkitt (pediatric, ≤18 years old) |
8q24.1 rearrangement |
5’/3’ MYC |
|
t(2;8)(p12;q24.1) |
IGK/MYC |
|
|
t(8;14)(q24.1;q32) |
MYC/IGH |
|
|
t(8;22)(q24.1;q11.2) |
MYC/IGL |
|
|
3q27 rearrangement |
3’/5’ BCL6 |
|
|
18q21 rearrangement |
3’/5’ BCL2 |
|
|
Diffuse large B-cell, Burkitt-like "double-hit" |
8q24.1 rearrangement |
5’/3’ MYC |
|
Reflex: t(2;8)(p12;q24.1) |
IGK/MYC |
|
|
Reflex: t(8;14)(q24.1;q32) |
MYC/IGH |
|
|
Reflex: t(8;22)(q24.1;q11.2) |
MYC/IGL |
|
|
Reflex: 3q27 rearrangement |
3’/5’ BCL6 |
|
|
Reflex: 18q21 rearrangement |
3’/5’ BCL2 |
|
|
Follicular |
18q21 rearrangement |
3’/5’ BCL2 |
|
3q27 rearrangement |
3’/5’ BCL6 |
|
|
Mantle Cell |
t(11;14)(q13;q32) |
CCND1/IGH |
|
Reflex: 11q13 rearrangement |
5’/3’ CCND1 |
|
|
Blastoid subtype only: deletion of 17p |
TP53/D17Z1 |
|
|
Blastoid subtype only: 8q24.1 rearrangement |
5’/3’ MYC |
|
|
MALT |
18q21 rearrangement |
5’/3’ MALT1 |
|
Splenic Marginal Zone |
Deletion of 7q |
D7Z1/7q32 |
|
Deletion of 17p |
TP53/D17Z1 |
|
Reference Values
An interpretive report will be provided.
Cautions
This test is not approved by the U.S. Food and Drug Administration and it is best used as an adjunct to existing clinical and pathologic information.
Bone marrow is the preferred sample type for this FISH test. If bone marrow is not available, a blood specimen may be used if there are malignant cells in the blood specimen (as verified by hematopathology).
This test is not appropriate for testing paraffin-embedded tissue samples from patients with B-cell lymphoma, see BLYMF / B-Cell Lymphoma, FISH, Tissue.
Day(s) Performed
Samples processed Monday through Sunday. Results reported Monday through Friday; 8 a.m. to 5 p.m. CST.
Report Available
7 daysPerforming Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
88271x2, 88291 – DNA probe, each (first probe set), Interpretation and report
88271x2 – DNA probe, each; each additional probe set (if appropriate)
88271x1 – DNA probe, each; coverage for sets containing 3 probes (if appropriate)
88271x2 – DNA probe, each; coverage for sets containing 4 probes (if appropriate)
88271x3 – DNA probe, each; coverage for sets containing 5 probes (if appropriate)
88274 w/modifier 52 – Interphase in situ hybridization, <25 cells, each probe set (if appropriate)
88274 – Interphase in situ hybridization, 25 to 99 cells, each probe set (if appropriate)
88275 – Interphase in situ hybridization, 100 to 300 cells, each probe set (if appropriate)