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HelpTest ID BMTF XX/XY in Opposite Sex Bone Marrow Transplantation, FISH
Useful For
Evaluating engraftment success by determining the proportion of donor and recipient interphase cells present in opposite sex bone marrow transplant recipients
Monitoring the proportion of host and recipient cells over time may be useful to identify significant clinical changes
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| _PBCT | Probe, +2 | No, (Bill Only) | No |
| _PADD | Probe, +1 | No, (Bill Only) | No |
| _PB02 | Probe, +2 | No, (Bill Only) | No |
| _PB03 | Probe, +3 | No, (Bill Only) | No |
| _IL25 | Interphases, <25 | No, (Bill Only) | No |
| _I099 | Interphases, 25-99 | No, (Bill Only) | No |
| _I300 | Interphases, ≥100 | No, (Bill Only) | No |
Testing Algorithm
This test only includes a charge for professional interpretation of results and does not include charges for probe application or analysis.
This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.
Method Name
Fluorescence In Situ Hybridization (FISH)
Reporting Name
XX/XY in Opposite Sex BMT, FISHSpecimen Type
VariesProvide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
Forms: If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request Form (T726) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf)
Advise Express Mail or equivalent if not on courier service.
Submit only 1 of the following specimens:
Specimen Type: Blood
Container/Tube: Green top (sodium heparin)
Specimen Volume: 7-10 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Other anticoagulants are not recommended and are harmful to the viability of the cells.
Specimen Type: Bone marrow
Container/Tube: Green top (sodium heparin)
Specimen Volume: 1-2 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Other anticoagulants are not recommended and are harmful to the viability of the cells.
Specimen Minimum Volume
Blood: 2 mL/Bone Marrow: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Clinical Information
Bone marrow transplantation (BMT) continues to be an important treatment for patients with malignant hematologic disorders and bone marrow failure syndromes. Conventional cytogenetic studies can be performed to evaluate a mixture of donor and recipient cells in opposite sex bone marrow transplants at a sensitivity of approximately 5%. Interphase FISH testing for X and Y chromosomes in opposite sex bone marrow transplant specimens results in an improved sensitivity of approximately 0.5%.
Reference Values
An interpretive report will be provided.
Cautions
This test is designed for opposite sex bone marrow transplants (BMTs) only; results are not useful for same sex bone marrow transplants.
Examination of the sex chromosome complement of interphase cells using FISH does not distinguish between malignant and normal cells. We strongly recommend using both FISH and cytogenetic studies to monitor patients.
A single X chromosome is sometimes lost in bone marrow cells of females, and the Y chromosome is sometimes lost in bone marrow cells of males, regardless of whether the specimen is from the donor, recipient, or a post-BMT patient.
Rare males may have an unusual Y chromosome that cannot be identified with these probes, but this finding should be readily apparent by analysis of metaphase cells using FISH.
Occasional patients may have chromosome polymorphisms that may hybridize with the Y probe, but this should be readily apparent by analysis of metaphase cells using FISH.
Day(s) Performed
Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m. to 5 p.m. CST.
Report Available
7 daysPerforming Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
88271x2, 88291 – DNA probe, each (first probe set), Interpretation and report
88271x2 – DNA probe, each; each additional probe set (if appropriate)
88271x1 – DNA probe, each; coverage for sets containing 3 probes (if appropriate)
88271x2 – DNA probe, each; coverage for sets containing 4 probes (if appropriate)
88271x3 – DNA probe, each; coverage for sets containing 5 probes (if appropriate)
88274 w/modifier 52 – Interphase in situ hybridization, <25 cells, each probe set (if appropriate)
88274 – Interphase in situ hybridization, 25 to 99 cells, each probe set (if appropriate)
88275 – Interphase in situ hybridization, 100 to 300 cells, each probe set (if appropriate)