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Test ID BRUC Brucella Culture

Useful For

Diagnosis of brucellosis

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
TISSR Tissue Processing No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed and charged.

Method Name

Conventional Culture Technique

Reporting Name

Brucella Culture

Specimen Type

Varies

Forms: If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).

 

Specimen source is required.

 

Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Bone marrow

Container/Tube: Sterile container

Specimen Volume: 1 mL

Specimen Stability Information: Ambient

 

Alternate:

Specimen Type: Abscess, respiratory specimen, spinal fluid, sterile body fluid, or tissue

Container/Tube: Sterile container                                                             

Specimen Volume: 1 mL

Specimen Stability Information: Refrigerated

Specimen Stability Information

Specimen Type Temperature Time
Varies Varies 24 hours

Clinical Information

Brucella are facultative intracellular gram negative staining bacilli capable of producing the disease "brucellosis" in humans. Human disease likely is acquired by contact with animals infected with the organism (Brucella abortus, Brucella suis, Brucella melitensis, and occasionally Brucella canis) either by direct contact or by ingestion of meat or milk. The signs and symptoms associated with brucellosis may include fever, night sweats, chills, weakness, malaise, headache, and anorexia. The physical examination may reveal lymphadenopathy and hepatosplenomegaly. A definitive diagnosis of brucellosis is made by recovering the organism from blood, fluid (including urine), or tissue specimens.

Reference Values

Negative (reported as positive or negative)

Cautions

Although Brucella are relatively resistant to adverse environmental conditions, excessive delay in the transport of material for culture to the laboratory may result in a lower yield.

 

Mayo Medical Laboratories does not perform species identification of Brucella; these isolates should be submitted to the State Health Department laboratory.

 

If a significant delay is expected, specimens should be refrigerated.

Day(s) Performed

Monday through Sunday; Continuously

Report Available

14 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87081-Brucella culture

87077-Identification by MALDI-TOF mass spec (if appropriate)

87153-Aerobe identification by sequencing (if appropriate)

87176-Tissue processing (if appropriate)

NY State Approved

Yes