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HelpTest ID BRUGM Brucella Antibody Screen, IgG and IgM, Serum
Useful For
Evaluating patients with suspected brucellosis
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| BRUTA | Brucella Ab, Agglutination, S | Yes | No |
Testing Algorithm
If Brucella antibody screen, IgG and IgM is positive or equivocal, then confirmation will be performed at an additional charge.
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Reporting Name
Brucella Ab Screen, IgG and IgM, SSpecimen Type
SerumContainer/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 1.5 mL
Forms: If not ordering electronically, complete, print, and send a Neurology Test Request Form-General (T732) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/neurology-request-form.pdf)
Specimen Minimum Volume
1.1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 14 days |
| Frozen | 14 days |
Clinical Information
Worldwide, brucellosis remains a major disease in humans and domesticated animals. Brucella infects goats (Brucella melitensis), cattle (Brucella abortus), swine (Brucella suis), and dogs (Brucella canis).(1) The disease has a limited geographic distribution. Few cases occur in the United States, with the bulk occurring in the Mediterranean region, Western Asia, and parts of Latin America and Africa.
Three species of Brucella commonly cause disease in humans: Brucella melitensis, Brucella suis, and Brucella abortus. The acute disease often presents with fever, chills, and malaise; the chronic form also causes abscesses in bone, brain, spleen, liver, and kidney.
Reference Values
IgG SCREEN
Negative (reported as positive, negative, or equivocal)
IgM SCREEN
Negative (reported as positive, negative, or equivocal)
Cautions
This test utilizes antigen derived from Brucella abortus strain W99. However, significant cross-reactivity exists for other Brucella species and, therefore, the assays should not be used to differentiate infection at the species level.
Brucella canis, a rare cause of brucellosis, may not be detected by this method.
Detection of specific IgM or IgG-class antibody to Brucella melitensis and Brucella suis by this method has not been determined.
Enzyme-linked immunosorbent assay (ELISA) tests are intended to be used as a screen only. Positive results should be followed up using an agglutination assay for confirmation. Results must be used in conjunction with symptoms, patient history, and other clinical findings.
Day(s) Performed
Monday, Wednesday, Friday; 9 a.m.
Report Available
Same day/1 day/ Additional days for agglutination test result if reflex testing is required.Performing Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions.CPT Code Information
86622 x 2-Brucella antibody, IgG and IgM
86622-Brucella total antibody, agglutination (if appropriate)