Necessary Information

The time the drug administration is started and completed, the patient's dose (mg every 6 hours), body weight (kg), and age (years) must be submitted with the specimens. Without infusion start time, exact time of specimen collection, dose, and body weight, the area under the curve and clearance cannot be calculated. Age is required for assessment of the correct dose per body weight.

A completed Busulfan Information: Mail-In Specimen Instructions (T559) is required.

Specimen Required

Four plasma specimens with different collection times (keep all specimens under 1 order) are required.

Collection Container/Tube: Green top (sodium heparin) (Plasma gel/PST are not acceptable)

Submission Container/Tube: Plastic vials

Specimen Volume: 1 mL

Collection Instructions:

1. The first specimen should be collected immediately after completion of the first intravenous infusion of 0.8 mg/kg busulfan.

2. Additional specimens should also be collected at 1 hour, 2 hours, and 4 hours after completion of infusion.

3. Label each specimen with exact time of collection.

4. Busulfan degrades quickly at ambient temperature. Specimens must be kept in wet ice slurry or refrigerated at 4° C. Specimens must be centrifuged within 2 hours after collection. Separate the plasma and transfer to individual 5-mL plastic vials, labeled with exact time of collection. Immediately freeze at -20° C.

Additional Information:

This test should only be ordered when the following criteria are met:

-Busulfan dosing protocol must be intravenous administration of 0.8 mg/kg doses every 6 hours over 4 days, for a total of 16 doses

-Specimens must be collected as described below:

-1 specimen collected immediately after completion of the first 2-hour IV infusion of busulfan

-1 specimen collected 1 hour after the infusion is completed

-1 specimen collected 2 hours after the infusion is completed

-1 specimen collected 4 hours after the infusion is completed and prior to the next infusion of busulfan