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Test ID C5B9 SC5b-9 Level Terminal Complement Complex, Plasma

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)


Ordering Guidance


If testing to evaluate C5b-9 in the context of atypical hemolytic uremic syndrome is desired, see AHUSD / Atypical Hemolytic Uremic Syndrome Complement Panel, Serum and Plasma.



Specimen Required


Patient Preparation:

1. Fasting: 8 hours, preferred but not required

2.  Do not collect specimens for at least 48 hours following plasma exchange.

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Lavender top (K2 EDTA)

Acceptable: Lavender top (K3 EDTA), light-blue top (sodium citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions:

1. Immediately after specimen collection, place the tube on wet ice.

2. Centrifuge between 1000 and 2000 x g for 10 minutes at 4° C and aliquot plasma into a plastic vial.

3. Within 30 minutes of centrifugation, freeze specimen. Specimen must be placed on dry ice if not frozen immediately.

NOTE: If a refrigerated centrifuge is not available, it is acceptable to use a room temperature centrifuge, provided the sample is kept on ice before centrifugation, and immediately afterward, the plasma is aliquoted and frozen.


Reporting Name

SC5b-9 Complement, P

Specimen Type

Plasma EDTA

Specimen Stability Information

Specimen Type Temperature Time
Plasma EDTA Frozen 14 days

Report Available

3 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

LOINC Code Information

Test ID Test Order Name Order LOINC Value
C5B9 SC5b-9 Complement, P 93244-2

 

Result ID Test Result Name Result LOINC Value
616921 SC5b-9 Complement, P 93244-2

Useful For

Detecting increased complement activation

Day(s) Performed

Varies

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

86160

Specimen Minimum Volume

0.75 mL

Reference Values

≤250 ng/mL