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Test ID CAMPY Helicobacter pylori Culture

Useful For

Recovery of Helicobacter pylori from gastric specimens for antimicrobial susceptibility testing of the organism

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
GID Bacteria Identification No No
TISSR Tissue Processing No No

Testing Algorithm

When this test is ordered, the reflex tests may be performed and charged.

 

See Helicobacter pylori Diagnostic Algorithm in Special Instructions.

Method Name

Conventional Culture Techniques

Reporting Name

Helicobacter pylori Culture

Specimen Type

Varies

Forms: If not ordering electronically, complete, print, and send a Microbiology Request Form (T244) (http://mayoweb.mayo.edu/sp-forms/mc0700-mc0799/mc0767.pdf).

 

Specimen must be received in laboratory within 48 hours of collection.

 

Specimen source is required; include the specific anatomic source.

 

Preferred:

Specimen Type: Gastric biopsy

Container/Tube: Sterile container

Specimen Volume: Entire collection

Collection Instructions: Acquire biopsied tissue; moisten with sterile saline.

 

Acceptable:

Specimen Type: Gastric brushing or gastric aspirate

Container/Tube: Sterile container

Specimen Volume: Entire collection

Collection Instructions: Aspirate contents with a syringe.

Specimen Minimum Volume

0.5 mL or 0.5 × 0.2 × 0.2-cm sized piece of tissue

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated 48 hours

Clinical Information

Helicobacter pylori is a spiral-shaped gram-negative bacterium that may cause chronic gastritis, peptic ulcer disease, or gastric neoplasia. In adults of industrialized countries, an estimated 0.5% of the susceptible population becomes infected each year, although the incidence has been decreasing over time. The organism may asymptomatically colonize humans. In suspected Helicobacter pylori-associated disease, the noninvasive stool antigen or urea breath test is recommended. If patients fail to respond to treatment and antimicrobial resistance is suspected, a gastric biopsy, gastric brushings, or gastric aspirate may be cultured to attempt to recover the organism for antimicrobial susceptibility testing to assess for resistance.

Reference Values

No growth after 7 days

Cautions

Culture-negative results may occur due to the fastidious nature of the organism. Delays in specimen transportation will decrease recovery of the organism. Culture should be set up as soon as possible following specimen collection. Antimicrobial therapy may render the culture negative.

 

Due to Helicobacter pylori's fastidious nature and slow growth, it may take 7 days to recover the organism and up to an additional 21 days to perform antimicrobial susceptibility testing when requested.

Day(s) Performed

Monday through Sunday; Continuously

Report Available

7 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87081-Helicobacter pylori culture

87077-Bacteria identification

87176-Tissue processing

NY State Approved

Yes