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HelpTest ID CFRC Bacterial Culture, Cystic Fibrosis, Respiratory
Useful For
Detection of aerobic bacterial pathogens from cystic fibrosis patient specimens
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| COMM | Identification Commercial Kit | No, (Bill Only) | No |
| RMALD | Ident by MALDI-TOF mass spec | No, (Bill Only) | No |
| GID | Bacteria Identification | No, (Bill Only) | No |
| ISAE | Aerobe Ident by Sequencing | No, (Bill Only) | No |
| REFID | Additional Identification Procedure | No, (Bill Only) | No |
| SALS | Serologic Agglut Method 1 Ident | No, (Bill Only) | No |
| EC | Serologic Agglut Method 2 Ident | No, (Bill Only) | No |
| SHIG | Serologic Agglut Method 3 Ident | No, (Bill Only) | No |
| STAP | Identification Staphylococcus | No, (Bill Only) | No |
| STRP | Identification Streptococcus | No, (Bill Only) | No |
| BLA | Beta Lactamase | No, (Bill Only) | No |
| KPNRP | KPC and NDM PCR | Yes, External; No Internal (KPND1 Bill Only) | No |
| HODGE | Carbapenamase-Modified Hodge Test | Yes, External; No Internal (HODGB Bill Only) | No |
| CARNP | Carbapenemase-Carba NP Test | Yes, External; No Internal (CARNB Bill Only) | No |
Testing Algorithm
When this test is ordered, the reflex tests may be performed and charged.
Method Name
Conventional Culture Technique
Reporting Name
Bacterial Culture, Cystic FibrosisSpecimen Type
VariesIf susceptibilities are desired when this test is ordered, also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, MIC. Each pathogen isolated will then be tested with the appropriate susceptibility panel. Charges are added for each susceptibility test performed.
Forms: If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).
Specimen must arrive within 48 hours of collection
Specimen source is required.
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Sputum, expectorated or induced
Container/Tube: Sterile container
Specimen Volume: Entire collection
Acceptable:
Specimen Type: Bronchial aspirate or washing, bronchoalveolar lavage, endotracheal, or tracheal
Container/Tube: Sterile container
Specimen Volume: Entire collection
Specimen Type: Throat swab
Container/Tube: Modified Stuart transport device
Specimen Volume: Entire specimen
Specimen Minimum Volume
2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Refrigerated | 48 hours |
Clinical Information
Life expectancy of patients with cystic fibrosis (CF) has increased steadily over the past 50 years, in large part due to improvements in the management of lung disease in this patient population. Still, chronic lung infection is responsible for 75% to 85% of deaths in patients with CF. Appropriate treatment for the causative organism can reduce morbidity and mortality.
The number of microbial species associated with CF lung disease is relatively limited. These include Pseudomonas aeruginosa (mucoid and nonmucoid), Staphylococcus aureus, Burkholderia cepacia complex, Stenotrophomonas maltophilia, other nonfermenting gram-negative rods, Haemophilus influenzae, and Streptococcus pneumoniae. Nontuberculous mycobacteria and Aspergillus species may also play a role in CF lung disease, in addition to common respiratory viruses. This culture, which is specifically designed for CF patients, utilizes conventional and additional selective media (compared to non-CF respiratory cultures) to isolate bacteria commonly associated with pulmonary disease in CF patients.
In selected centers, lung transplantation is performed on CF patients. This test is appropriate for lung transplant patients with underlying CF because they can continue to harbor the same types of organisms as they did pretransplantation. CF patients may be colonized or chronically infected by these organisms over a long period of time.
Reference Values
No growth or usual flora
Identification of probable pathogens
Cautions
Some bacterial agents that cause lower respiratory infections (eg, mycobacteria, Legionella species, Mycoplasma pneumoniae) are not detected by this assay and require special procedures. If the bacterial culture is negative, clinicians should consider additional testing to detect other bacterial, viral, or fungal agents.
Results must be interpreted in conjunction with clinical findings and previous culture results.
Day(s) Performed
Monday through Sunday; Continuously
Report Available
5 daysPerforming Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87070-Bacteria, culture, cystic fibrosis, respiratory
87077-Identification commercial kit (if appropriate)
87077-Ident by MALDI-TOF mass spec (if appropriate)
87077-Bacteria Identification (if appropriate)
87077-Additional Identification procedure (if appropriate)
87077-Identification Staphylococcus (if appropriate)
87077-Identification Streptococcus (if appropriate)
87147 x 5-Serologic agglut method 1 ident (if appropriate)
87147-Serologic agglut method 2 ident (if appropriate)
87147 x 4-Serologic agglut method 3 ident (if appropriate)
87153-Aerobe Ident by sequencing (if appropriate)
87185-Beta lactamase (if appropriate)
87185-Carbapenemase detection, Carba NP test (if appropriate)
87185-Carbapenemase detection, modified Hodge test (if appropriate)
87798 x 2-KPC and NDM PCR (if appropriate)