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Test ID CTBID Culture Referred for Identification, Mycobacterium and Nocardia

Infectious

Useful For

Rapid identification to the species level for Mycobacterium species, Nocardia species, and other aerobic actinomycete genera and species from pure culture isolates

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RMALM Id MALDI-TOF Mass Spec AFB No, (Bill Only) No
RTBSP Id, Mtb Speciation, PCR No, (Bill Only) No
TBMP Mycobacteria Probe Ident No, (Bill Only) No
TBPB Mycobacteria Probe Ident Broth No, (Bill Only) No
ISMY ID by 16S Sequencing No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex test may be performed and charged. All mycobacteria and Nocardia (including aerobic actinomycetes) submitted will be identified and billed as appropriate.

 

See Culture Referred for Identification Mycobacterium Algorithm in Special Instructions.

Method Name

Nucleic Acid Probe/16S rDNA Sequencing/MALDI-TOF Mass Spectrometry/Rapid PCR

 

Nucleic acid probes used for identification, when applicable, include those for Mycobacterium avium-intracellulare complex, Mycobacterium gordonae, and Mycobacterium tuberculosis complex. MALDI-TOF Mass Spectrometry and/or 16S rDNA sequencing is used for identification, when applicable, for slowly and rapidly growing Mycobacterium species and aerobic actinomycetes (including Nocardia species and Streptomyces species). The Mycobacterium tuberculosis complex will be further identified to the species level upon request, using rapid PCR.

Reporting Name

Culture Refer for ID, Mycobacterium

Specimen Type

Varies

Specimen Type: Organism in pure culture

Container/Tube: Middlebrook (7H10 or 7H11) or Lowenstein-Jensen medium slant or in broth (eg, Mycobacteria Growth Indicator Tube [7H9] broth)

Specimen Volume: Isolate

Collection Instructions:

1. Organism must be in pure culture, actively growing. Do not submit mixed cultures.

2. Place specimen in a large infectious container (Supply T146) and label as an etiologic agent/infectious substance.

Additional Information:

1. Specimen source is required.

2. Isolate description is required: Gram stain reaction, morphology, tests performed.

3. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.

Forms: If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Clinical Information

There are over 170 recognized species of mycobacteria and more than 100 Nocardia species. Many of these species are human pathogens and, therefore, identification to the species level is important to help guide patient care. In addition, there are other aerobic actinomycete genera that can be human pathogens including, but not limited to, Tsukamurella, Rhodococcus, and Gordonia species.

 

Nucleic acid hybridization probes are utilized that identify specific ribosomal RNA sequences of Mycobacterium tuberculosis complex, Mycobacterium avium complex, and Mycobacterium gordonae. Other Mycobacteria species, Nocardia species and other aerobic actinomycete genera are identified using MALDI-TOF mass spectrometry or nucleic acid sequencing of a 500-base pair region of the 16S ribosomal RNA gene.

Reference Values

Not applicable

Cautions

If the organism is received in mixed culture or contaminated, the report may be delayed or identification may not be possible.

Day(s) Performed

Monday through Sunday; Continuously

Report Available

60 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

Culture Referred for Identification, Mycobacterium

87158-Identification of mycobacteria by other methods (if appropriate)

87118 -Id MALDI-TOF Mass Spec AFB (if appropriate)

87153-Mycobacteria Identification by Sequencing (if appropriate)

87150-Mycobacteria Probe Ident, Solid (if appropriate)

87150-Mycobacteria Probe Ident, Broth (if appropriate)

87150-Id, Mtb Speciation, PCR (if appropriate)

NY State Approved

Yes