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HelpTest ID CTBID Culture Referred for Identification, Mycobacterium and Nocardia, Varies
Infectious
Shipping Instructions
1. See Infectious Specimen Shipping Guidelines in Special Instructions.
2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.
Necessary Information
1. Specimen source is required.
2. Isolate description is required: Gram stain reaction, morphology, tests performed.
Specimen Required
Specimen Type: Organism in pure culture
Supplies: Infectious Container, Large (T146)
Container/Tube: Middlebrook (7H10 or 7H11) or Lowenstein-Jensen medium slant or in broth (eg, Mycobacteria Growth Indicator Tube [7H9] broth)
Specimen Volume:
Visible growth of isolate on solid media
Isolate in broth media: ≥3 mL
A minimum volume of 3 mL is recommended in order to perform all initial testing, this may include: stains, sub-culture media, nucleic acid probes, and any additional testing that may be required to determine the identification. If the broth sample volume is <3 mL, initial testing may be limited, and increased turnaround time is likely.
Collection Instructions: Organism must be in pure culture, actively growing. Do not submit mixed cultures.
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
Secondary ID
80278Useful For
Rapid identification to the species level for Mycobacterium species, Nocardia species, and other aerobic actinomycete genera and species from pure culture isolates
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| RMALM | Id MALDI-TOF Mass Spec AFB | No, (Bill Only) | No |
| RTBSP | Id, Mtb Speciation, PCR | No, (Bill Only) | No |
| TBMP | Mycobacteria Probe Ident | No, (Bill Only) | No |
| TBPB | Mycobacteria Probe Ident Broth | No, (Bill Only) | No |
| ISMY | ID by 16S Sequencing | No, (Bill Only) | No |
| LCTB | Id, MTB complex Rapid PCR | No, (Bill Only) | No |
Testing Algorithm
When this test is ordered, the reflex test may be performed at an additional charge. All mycobacteria and Nocardia (including aerobic actinomycetes) submitted will be identified and billed as appropriate.
See Culture Referred for Identification Mycobacterium and Nocardia Algorithm.
Special Instructions
Method Name
Nucleic Acid Probe/16S rDNA Sequencing/Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)/Rapid PCR
Reporting Name
Culture Refer for ID, MycobacteriumSpecimen Type
VariesSpecimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Clinical Information
There are approximately 200 recognized species of mycobacteria and more than 100 Nocardia species. Many of these species are human pathogens and, therefore, identification to the species level is important to help guide patient care. In addition, there are other aerobic actinomycete genera that can be human pathogens including, but not limited to, Tsukamurella, Rhodococcus, and Gordonia species.
Nucleic acid hybridization probes are utilized that identify specific ribosomal RNA sequences of Mycobacterium tuberculosis complex, Mycobacterium avium complex, and Mycobacterium gordonae. Other Mycobacteria species, Nocardia species and other aerobic actinomycete genera are identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) or nucleic acid sequencing of a 500-base pair region of the 16S ribosomal RNA gene.
Reference Values
Not applicable
Cautions
If the organism is received in mixed culture or contaminated, the report may be delayed or identification may not be possible.
Day(s) Performed
Monday through Sunday
Report Available
60 to 70 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
Culture Referred for Identification, Mycobacterium
87118-Identification of mycobacteria
87158-Identification of mycobacteria by other methods (if appropriate)
87118 -Id MALDI-TOF Mass Spec AFB (if appropriate)
87153-Mycobacteria Identification by Sequencing (if appropriate)
87150-Mycobacteria Probe Ident, Solid (if appropriate)
87150-Mycobacteria Probe Ident, Broth (if appropriate)
87150-Id, Mtb Speciation, PCR (if appropriate)
87150- Id, MTB complex Rapid PCR (if appropriate)