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Test ID DCTM Direct Coombs, Blood

Useful For

Demonstrating in vivo coating of RBCs with IgG or the complement component C3d in the following settings:

-Autoimmune hemolytic anemia

-Hemolytic transfusion reactions

-Drug-induced hemolytic anemia

Method Name

Direct Antiglobulin Test/Standard AABB

Reporting Name

Direct Coombs, B

Specimen Type

Whole Blood EDTA

Specimen must arrive within 48 hours of draw.

 

Container/Tube: Pink top (EDTA)

Specimen Volume: 6 mL

Additional Information:

1. Diagnosis, medication, and recent transfusion history are required.

2. Monospecific anti-C3, and anti-IgG direct Coombs can be ordered separately using the following codes: MDCC3 / Monospecific Direct Coombs C3, Blood and MDCG / Monospecific Direct Coombs IgG, Blood.

Specimen Minimum Volume

3 mL of blood in a 6-mL pink EDTA Infants: Heelstick 2 to 3 drops of blood in 0.9% saline

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood EDTA Ambient (preferred) 48 hours
  Refrigerated  48 hours

Clinical Information

IgG antibody or complement components secondary to the action of IgM antibody may be present on the patient's own RBCs or on transfused RBCs.

Reference Values

Negative

If positive, reaction is graded (micro positive to 4+).

Cautions

Autoimmune hemolytic anemia can occasionally occur with a negative direct Coombs test.

 

The direct Coombs test can be positive in some apparently healthy individuals.

Day(s) Performed

Monday through Sunday; Continuously

Report Available

Same day/1 day

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86880

NY State Approved

Yes