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Test ID DIS2 HLA Class II Molecular Typing Disease Association

Useful For

Determining HLA Class II compatibility on specimens from bone marrow and solid organ transplant candidates and their donors

Method Name

Polymerase Chain Reaction (PCR)/Sequence-Specific Oligonucleotide Probes (SSO)

(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Reporting Name

HLA ClassII Mol Typing DiseaseAssoc

Specimen Type

Whole Blood ACD-B

Container/Tube: Yellow top (ACD solution B)

Specimen Volume: 6 mL

Collection Instructions: Do not transfer blood to other containers.

Additional Information: Specimen acceptability is based on extracted DNA concentration and not sample age.

Forms: If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request Form (T726)

(http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf)

Specimen Minimum Volume

3 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood ACD-B Refrigerated (preferred)
  Ambient 

Clinical Information

Human leukocyte antigens (HLA) are regulators of the immune response that play a key role in transplantation.

                                                                                                           

Sequence-specific oligonucleotides are designed to provide low-to-medium resolution for HLA Class II (DR and DQ) typing. Low-to-medium resolution defines the typing at the antigen level. This is in contrast to high-resolution typing, which defines typing at the allele (molecular) level and is used primarily for typing donor-recipient pairs for unrelated bone marrow transplantation.

Reference Values

Not applicable

Cautions

No significant cautionary statements

Day(s) Performed

Monday through Friday; 7:30 a.m.-5 p.m.

Report Available

5 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

81375-HLA Class II typing, low resolution (eg, antigen equivalents); HLA-DRB1/3/4/5 and -DQB1

NY State Approved

Yes