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Test ID DISI HLA Class I Molecular Typing Disease Association

Useful For

Determining class I HLA antigens on specimens for transplant candidates and their donors or those who have become refractory to platelet transfusions

Method Name

Polymerase Chain Reaction (PCR)/Sequence-Specific Oligonucleotide Probes (SSO)

(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Reporting Name

HLA Class I Mol Typing DiseaseAssoc

Specimen Type

Whole Blood ACD-B

Container/Tube: Yellow top (ACD solution B)

Specimen Volume: 6 mL                           

Collection Instructions: Do not transfer blood to other containers. 

Additional Information: Specimen acceptability is based on extracted DNA concentration and not sample age.

Forms: If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request Form (T726)

(http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf)

Specimen Minimum Volume

3 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood ACD-B Refrigerated (preferred)
  Ambient 

Clinical Information

Human leukocyte antigens (HLA) are regulators of the immune response that play a key role in transplantation. HLA class I typing is most frequently applicable to organ transplant donor/recipient matching, provision of HLA-matched platelets for alloimmunized refractory patients, and for a small number of disease associations.

 

Class I HLA antigens include A, B, and C loci. This assay is designed to provide low-to-medium resolution for HLA class I typing (A, B, C). Low-to-medium resolution defines the typing at the antigen level. This is in contrast to high-resolution typing, which defines typing at the allele (molecular) level and is used primarily for typing donor/recipient pairs for unrelated bone marrow transplantation.

Reference Values

Not applicable

Cautions

No significant cautionary statements

Day(s) Performed

Monday through Friday; 7:30 a.m.-5 p.m.

Report Available

5 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

81372-HLA class I typing, low resolution (eg, antigen equivalents); complete (ie, HLA-A, -B and -C)

NY State Approved

Yes