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HelpTest ID DMTBR Mycobacterium tuberculosis Complex, Molecular Detection of Drug Resistance Markers, Next-Generation Sequencing
Ordering Guidance
This test is used to identify the drug resistance variants in an M tuberculosis complex culture isolate.
Shipping Instructions
1. For shipping information see Infectious Specimen Shipping Guidelines
2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.
Necessary Information
Specimen source and organism identification are required.
Specimen Required
Supplies: Infectious Container, Large (T146)
Specimen Type: Mycobacterium tuberculosis complex isolate growing in pure culture
Container/Tube:
Preferred: Solid slant medium (eg. Middlebrook 7H10, 7H11 or Lowenstein Jensen agar)
Acceptable: Broth medium (eg. Mycobacteria Growth Indicator Tube, 7H9 broth, BACT/ALERT MP or VersaTREK bottle). Note: Broth specimens will require subculture which will delay results.
Specimen Volume:
Solid slant medium: Isolate with visible growth
Broth medium: Greater than or equal to 3 mL of broth culture
Collection Instructions:
1. Organism must be in pure culture, actively growing. Do not submit mixed cultures.
2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.
3. Turnaround time for results may be delayed if subculture is needed to ensure purity.
Reporting Name
M tuberculosis drug resistance, NGSSpecimen Type
VariesSpecimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Report Available
7 to 14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DMTBR | M tuberculosis drug resistance, NGS | 94053-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| TBSS | Isolate from Specimen Source | 31208-2 |
| TBDI | Organism Identification | 42803-7 |
| 623237 | rpoB | 94065-0 |
| 623238 | inhA | 94055-1 |
| 623239 | fabG1 | 94056-9 |
| 623240 | katG | 94054-4 |
| 623241 | ahpC | 94057-7 |
| 623242 | ethA | 72866-7 |
| 623243 | pncA | 94059-3 |
| 623244 | embB | 94058-5 |
| 623245 | gidB | 94061-9 |
| 623246 | rpsL | 94063-5 |
| 623247 | rrs | 94062-7 |
| 623248 | eis | 94064-3 |
| 623249 | tlyA | In Process |
| 623250 | gyrA | 94060-1 |
| 623251 | gyrB | 101576-7 |
| 623252 | rrl | In Process |
| 623253 | rplC | In Process |
| 623254 | rv0678 | In Process |
| 623255 | rifampin (RIF) Interpretation | 46244-0 |
| 623256 | isoniazid (INH) Interpretation | In Process |
| 623257 | pyrazinamide (PZA) Interpretation | 46245-7 |
| 623258 | ethambutol (EMB) Interpretation | 46247-3 |
| 623259 | streptomycin (SM) Interpretation | In Process |
| 623260 | amikacin (AMI) Interpretation | In Process |
| 623261 | kanamycin (KAN) Interpretation | In Process |
| 623262 | capreomycin (CAP) Interpretation | In Process |
| 623263 | fluoroquinolones (FQ) Interpretation | In Process |
| 623264 | ethionamide (ETH) Interpretation | 72866-7 |
| 623265 | linezolid (LIN) Interpretation | In Process |
| 623266 | bedaquiline (BDQ) Interpretation | In Process |
| 623267 | clofazimine (CFZ) Interpretation | In Process |
| 623508 | Footnotes | 48767-8 |
Useful For
Molecular detection of drug resistance variants in culture isolates of the Mycobacterium tuberculosis complex
May provide a more rapid detection of drug resistance than phenotypic, broth-based testing for selected gene targets
Aiding in the resolution of discrepant results obtained using phenotypic methods
Testing for M tuberculosis complex isolates that are not sufficiently viable to allow for culture-based testing
Day(s) Performed
Monday through Friday
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87153 - M tuberculosis drug resistance, NGS
Specimen Minimum Volume
See Specimen Required
Reference Values
Not applicable
Method Name
Next-Generation Sequencing (NGS)
Testing Algorithm
Next-Generation sequencing (NGS) of Mycobacterium tuberculosis complex isolates is performed followed by evaluation of selected genes of interest for the presence of well-characterized, drug resistance-conferring variants.