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Test ID EBF Electrophoresis, Protein, Body Fluid

Useful For

Monitoring patient's body fluid proteins

 

Aiding in the diagnosis of monoclonal gammopathies, when used in conjunction with immunofixation of the patient's serum

 

Detecting oligoclonal banding in spinal fluid (the preferred test for detecting oligoclonal bands in spinal fluid is OLIG / Oligoclonal Banding, Serum and Spinal Fluid)

Profile Information

Test ID Reporting Name Available Separately Always Performed
FLD4 Fluid Type No Yes
TP1 Protein, Total Yes, (TPBF) Yes
ELPBF Protein Electrophoresis, BF No Yes

Method Name

Agarose Gel Electrophoresis

Reporting Name

Electrophoresis, Protein, BF

Specimen Type

Body Fluid

Container/Tube: Sterile container

Specimen Volume: 10 mL

Collection Instructions: Centrifuge to remove any cellular material.

Additional Information: Indicate specimen source; acceptable body fluid types are peritoneal (abdominal, ascites, paracentesis, peritoneal), pleural (chest, thoracentesis, pleural), drain (drainage, Jackson-Pratt (JP) drain), pericardial, spinal (CSF or ventricular), synovial (joint, knee, elbow), and vitreous fluids

Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time
Body Fluid Refrigerated (preferred) 7 days
  Frozen  14 days
  Ambient  72 hours

Clinical Information

Body fluid proteins can be grouped into 5 fractions by protein electrophoresis (PEL):

-Albumin

-Alpha-1 Globulin

-Alpha-2 Globulin

-Beta Globulin

-Gamma Globulin

The concentration of these fractions and the electrophoretic pattern may identify abnormalities in the levels of the various fractions.

 

Protein electrophoresis alone is not considered an adequate screening for body fluid or serum monoclonal gammopathies.

Reference Values

Not applicable

Cautions

Hemolysis may augment the beta fraction.

 

Penicillin may split the albumin band.

 

Radiographic agents may produce an uninterpretable pattern.

Day(s) Performed

Monday through Saturday; 12 p.m.

Report Available

1 day

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

See Individual Test IDs

CPT Code Information

84166

NY State Approved

Yes