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HelpTest ID EEEVP Red Blood Cell (RBC) Enzyme Evaluation
Useful For
Identifying defects of red cell enzyme metabolism
Evaluating patients with hemolytic anemia
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| EEEV | Erythrocyte Enzyme Interpretation | No | Yes |
| G6PD | G-6-PD, QN, RBC | Yes | Yes |
| PK | Pyruvate Kinase, RBC | Yes | Yes |
| GPI | Glucose Phosphate Isomerase, B | Yes | Yes |
| HEXK | Hexokinase, B | No | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| GLTI | Glutathione, B | No | No |
| RBCE | Reflexed RBC Enzymes | No | No |
Testing Algorithm
This is a consultative evaluation in which the case will be evaluated at Mayo Medical Laboratories, the appropriate tests performed at an additional charge, and the results interpreted.
Note: RBCE / Reflexed RBC Enzymes, Blood includes: adenosine deaminase, adenylate kinase, phosphofructokinase, phosphoglycerate kinase, triosephosphate isomerase, and pyrimidine 5'nucleotidase.
Method Name
EEEV: Consultative Interpretation
G6PD, GPI, PK, GLTI, HEXK, RBCE: Kinetic Spectrophotometry (KS)
Reporting Name
RBC Enzyme EvaluationSpecimen Type
Whole Blood ACD-BContainer/Tube: Yellow top (ACD solution B)
Specimen Volume: 12 mL
Collection Instructions: Do not transfer blood to other containers.
Forms: If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf)
Specimen Minimum Volume
5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood ACD-B | Refrigerated | 8 days |
Clinical Information
All enzyme defects, including erythrocyte enzyme errors, are inherited; some are sex-linked and located on the X chromosome. Some family members have no hematologic abnormalities, while others have a hemolytic anemia. For a number of RBC enzyme defects (eg, deficiencies of hexokinase, glucose phosphate isomerase, pyruvate kinase), the sole clinical manifestation is hemolytic anemia. Glucose-6-phosphate dehydrogenase deficiency is the most common metabolic error of the red cell and presents with acute hemolytic anemia in response to oxidant stress (eg, drugs, acute infections, fava bean ingestion).
This is a consultative evaluation looking at red cell enzyme defects as the cause for early red cell destruction.
Reference Values
Definitive results and an interpretive report will be provided.
Cautions
No significant cautionary statements
Day(s) Performed
Monday through Friday; Varies
Report Available
2-10 days (not reported Saturday or Sunday)Performing Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
82955-G-6-PD
84087-Glucose phosphate isomerase
84220-Pyruvate kinase
82657-Hexokinase
82978-Glutathione (if appropriate)
83915-RBC Enzymes (if appropriate)