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HelpTest ID EOSU Eosinophils, Urine
Useful For
Investigation of possible acute interstitial nephritis
Method Name
Hansel Stain
Reporting Name
Eosinophils, USpecimen Type
UrineContainer/Tube: Unstained slide
Specimen Volume: 1 slide
Collection Instructions:
1. Collect a random urine specimen.
2. Spin 10 mL of a random urine collection in centrifuge.
3. Pour off supernatant. Assess urine sediment for presence of white blood cells (WBC). If no WBCs are seen, the eosinophil test can’t be performed. If WBCs are present, prepare slides using the spun sediment.
4. Unstained slide should be prepared within 2 hours of collection.
5. Centrifuge urine in cytospin centrifuge at speed of 750 rpm for 5 minutes.
6. Guidelines for slide preparation. Use the sediment to prepare slides as follows:
a. 150 mcL of sample for 1 to 3 WBC/high power field (hpf).
b. 100 mcL of sample for moderate to normal sediment (<50 cell/hpf).
c. 50 mcL of sample for heavier sediment (>50 cells/hpf).
d. 25 mcL for packed fields of sediment.
Additional Information:
1. Stained cytospin slides on Mayo Medical Laboratories patients are retained for 7 days.
2. Call Mayo Medical Laboratories at 800-533-1710 or 507-266-5700 with questions.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Urine | Refrigerated (preferred) | 14 days |
| Ambient | 14 days | |
| Frozen | 14 days |
Clinical Information
Eosinophils are white blood cells that normally do not appear in urine. The presence of eosinophils in the urine is seen in acute interstitial nephritis, which is caused by an allergic reaction, typically to drugs.
Reference Values
None seen
Cautions
No significant cautionary statements.
Day(s) Performed
Monday through Sunday; Continuously
Report Available
Same day/1 dayPerforming Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
89050