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HelpTest ID ETGX Ethyl Glucuronide Confirmation, Chain of Custody, Urine
Useful For
Monitoring abstinence in clinical and justice system settings using ethyl blucuronide and ethyl sulfate as direct biomarkers or metabolites of ethanol
This chain-of-custody test is intended to be used in a setting where the test results can be used definitively to make a diagnosis.
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| COCH | Chain of Custody Processing | No | Yes |
| ADLTX | Adulterants Survey, CoC, U | Yes | Yes |
Testing Algorithm
Adulterants testing will be performed on all chain-of-custody urine samples as per regulatory requirements.
Method Name
ETGX: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
ADLTX: Spectrophotometry (SP)
Reporting Name
Ethyl Glucuronide Conf, CoC, USpecimen Type
UrineSpecimen Volume: 20 mL
Collection Instructions: Collect specimen in the provided container, seal, and submit with the associated documentation to satisfy the legal requirements for chain-of-custody testing.
Additional Information: Submitting <20 mL will compromise our ability to perform all necessary testing.
Forms: Chain-of-Custody Request Form is included in the Chain-of-Custody Kit (T282). A copy of this form is also available at http://www.mayomedicallaboratories.com/it-mmfiles/chain-of-custody-request-form.pdf.
Specimen Minimum Volume
2.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Urine | Refrigerated (preferred) | 28 days |
| Frozen | 28 days | |
| Ambient | 72 hours |
Clinical Information
Ethyl glucuronide and ethyl sulfate are minor metabolites of ethanol that are detectable in body fluids following alcohol consumption and, less commonly, following extraneous exposure. Ethyl glucuronide (EtG) and ethyl sulfate (EtS) are direct biomarkers or metabolites of ethanol. EtG and EtS can be detected up to 5 days in urine using a cutoff of 500 ng/mL.(1)
Chain of custody is a record of the disposition of a specimen to document who collected it, who handled it, and who performed the analysis. When a specimen is submitted in this manner, analysis will be performed in such a way that it will withstand regular court scrutiny. Chain of custody is required whenever the results of testing could be used in a court of law. Its purpose is to protect the rights of the individual contributing the specimen by demonstrating that it was under the control of personnel involved with testing the specimen at all times; this control implies that the opportunity for specimen tampering would be limited.
Reference Values
Negative
Cutoff concentrations:
Ethyl Glucuronide: 500 ng/mL
Cautions
Please note that incidental exposure to alcohol in many daily use products (ie, hand sanitizers, mouthwash) may result in detectable levels of ethyl glucuronide (EtG) and ethyl sulfate (EtS).
In addition, upper respiratory infections as well as beta glucuronidase hydrolysis may lower levels of EtG, but do not seem to affect EtS.(2)
EtG/EtS results should be interpreted in the context of all available clinical and behavioral information.
Day(s) Performed
Monday through Friday
Report Available
Same day/1 dayPerforming Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
80302
80321 (if appropriate)
G0479 (if appropriate)