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HelpTest ID EXHBM Hematologic Disorders, DNA/RNA Extract and Hold, Bone Marrow
Useful For
Reserving nucleic acid on any specimen for which molecular analysis may be necessary at a future date, ensuring that adequate material for testing is available
Testing Algorithm
DNA and total RNA will be extracted from the specimen and stored at -80 degrees C for 1 year from the date the extraction process was performed. A report of "Performed" will be sent and a $75.00 processing fee will be assessed. To request molecular testing from the Molecular Hematopathology Laboratories test menu, call 800-533-1710. Additional charges will be assessed for testing performed.
Method Name
Nucleic Acid Extraction
Reporting Name
DNA and RNA, Extract and Hold BMSpecimen Type
Bone MarrowSpecimen must arrive within 72 hours of draw. Draw and package specimen as close to shipping time as possible.
Collection Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: ACD
Specimen Volume: 2 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Send specimen in original tube.
Additional Information:
1. Date of draw is required.
2. To request molecular testing from the Molecular Hematopathology Laboratory, call 800-533-1710. Additional charges will be assessed for testing performed.
Forms: If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request Form (T726) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf)
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Bone Marrow | Ambient (preferred) | 72 hours |
| Refrigerated | 72 hours |
Clinical Information
It is frequently useful to obtain nucleic acid from clinical specimens containing a hematopoietic neoplasm at the time of diagnosis, so that appropriate material is available for molecular analysis should subsequent testing be required. For example, when a diagnosis of acute myelogenous leukemia is made, there is a delay before karyotype information, which determines whether testing for molecular prognostic markers is necessary, is available. After this delay, the diagnostic sample is usually no longer available or the nucleic acid has degraded to such an extent that it is no longer adequate for testing. Thus, it is useful to obtain nucleic acid on such specimens promptly at diagnosis and retain it until it is known whether additional testing is necessary.
Reference Values
Not applicable
Cautions
The nucleic acid will be held at -80° C for 1 year from the time of extraction. The nucleic acid may only be used for suitable tests offered by the Molecular Hematopathology Laboratory at Mayo Clinic. Nucleic acid cannot be shipped to another institution for testing and cannot be released for research use.
Only bone marrow will be processed. Paraffin-embedded material and fresh, solid tissue will not be routinely accepted at this time.
Day(s) Performed
Monday through Friday
Specimens received Saturday or Sunday will be processed on Monday.
Report Available
2 daysPerforming Laboratory
Mayo Medical Laboratories in Rochester