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HelpTest ID FCFT Cortisol, Free and Total
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LS/MS/MS), Equilibrium Dialysis
Reporting Name
Cortisol, Free and TotalSpecimen Type
VariesSubmit only one of the following:
Serum
Draw blood in a plain red-top tube(s), serum gel tube(s) is acceptable. Spin down and send 2 mL of serum refrigerated in a plastic vial.
Plasma
Draw blood in an EDTA (Lavender top) or PPT potassium EDTA (white top) tube(s). Spin down and send 2 mL of plasma refrigerate in a plastic vial.
Specimen Minimum Volume
0.7 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Refrigerated (preferred) | 7 days |
| Ambient | 48 hours | |
| Frozen | ||
Reference Values
Adult Reference Ranges for Cortisol, Total:
Males and Females: 8 – 10 AM 4.6 – 20.6 mcg/dL
4 – 6 PM 1.8 – 13.6 mcg/dL
Cortisol Response to ACTH:
Adult Males and Females
Peak >20.0 mcg/dL
Peak >16.0 mcg/dL after IM injection
Adult Reference Ranges for Cortisol, Free:
8:00 – 10:00 AM 0.07 – 0.93 mcg/dL
4:00 – 6:00 PM 0.04 – 0.45 mcg/dL
10:00 – 11:00 PM 0.04 – 0.35 mcg/dL
Day(s) Performed
Monday through Friday
Report Available
5 daysPerforming Laboratory
Quest Diagnostics Nichols InstituteCPT Code Information
82530
82533