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HelpTest ID FGEN Fungal Culture, Routine
Useful For
Diagnosing fungal infections from specimens other than blood, skin, hair, nail, and vagina (separate tests are available for these specimen sites)
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| API | Yeast Ident Panel C | No, (Bill Only) | No |
| D2F | D2 Fungal Sequencing Identification | No, (Bill Only) | No |
| FUNA | Fungal Ident Panel A | No, (Bill Only) | No |
| FUNB | Fungal Ident Panel B | No, (Bill Only) | No |
| GT | Yeast Ident Panel A | No, (Bill Only) | No |
| LCCI | Ident Rapid PCR Coccidioides | No, (Bill Only) | No |
| LCHB | Id, Histoplasma/Blastomyces PCR | No, (Bill Only) | No |
| RAT1 | Yeast Ident panel B | No, (Bill Only) | No |
| RMALF | Id MALDI-TOF Mass Spec Fungi | No, (Bill Only) | No |
| RRU | Yeast Ident Panel D | No, (Bill Only) | No |
| TISSR | Tissue Processing | No, (Bill Only) | No |
| RMALY | Id MALDI-TOF Mass Spec Yeast | No, (Bill Only) | No |
Testing Algorithm
When this test is ordered, the reflex tests may be performed and charged.
Method Name
Conventional agar culture technique with identification by macroscopic and microscopic morphology, D2 rDNA gene sequencing, real-time polymerase chain reaction (rtPCR), or MALDI-TOF mass spectrometry. Dimorphic pathogen identification is confirmed using molecular methods (ie, D2 rDNA gene sequencing, rtPCR or MALDI-TOF mass spectrometry).
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)
Reporting Name
Fungal Culture, RoutineSpecimen Type
VariesForms: If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).
Specimen should arrive within 24 hours of collection.
Specimen source is required.
Specimen Type: Body fluid
Container/Tube: Sterile container
Specimen Volume: Entire collection
Specimen Type: Bone marrow
Container/Tube: Sterile container
Specimen Volume: Entire collection
Specimen Type: Fresh tissue or stool
Container/Tube: Sterile container
Specimen Volume: Pea sized
Collection Instructions: Tissue should be placed in small amount of sterile saline or sterile water.
Specimen Type: Respiratory specimen
Container/Tube: Sterile container
Specimen Volume: Entire collection
Specimen Type: Swab
Fresh tissue or body fluid is the preferred specimen type instead of a swab specimen.
Sources: Dermal, ear, mouth, ocular, throat, or wound
Container/Tube: Culture transport swab (non-charcoal) Culturette
Specimen Volume: Swab
Collection Instructions:
1. Before collecting specimen, wipe away any excessive amount of secretion and discharge, if appropriate.
2. Obtain secretions or fluid from source with sterile swab.
3. If smear and culture are requested or both a bacterial culture and fungal culture are requested, collect a second swab to maximize test sensitivity.
Specimen Type: Urine
Container/Tube: Sterile container
Specimen Volume: 2 mL
Collection Instructions: Collect a random urine specimen.
Specimen Minimum Volume
Bone Marrow or Body Fluid: 1 mL/Respiratory Specimen: 1.5 mL/Tissue: pea-sized piece
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Refrigerated (preferred) | 7 days |
| Ambient | 7 days |
Clinical Information
Many fungi in the environment cause disease in immunocompromised human hosts. Accordingly, the range of potential pathogenic fungi has increased as the number of immunosuppressed individuals (eg, persons with AIDS, patients receiving chemotherapy or transplant rejection therapy) has increased. Isolation and identification of the infecting fungus in the clinical laboratory can help guide patient care.
Reference Values
Negative
If positive, fungus will be identified.
Cautions
For optimal recovery of organisms, sufficient specimen should be transported within 24 hours of collection.
Fungi can be pathogens, colonizers, or contaminants. Correlation of the patient clinical condition with culture results is necessary.
Nocardia and the other aerobic actinomycetes are not fungi and, therefore, a fungal culture should not be ordered. These organisms grow well on mycobacterial medium and therefore ordering a mycobacterial culture is recommended when infection with this group of organisms is suspected.
Day(s) Performed
Monday through Sunday; Continuously
Report Available
24 days/Positive cultures reported when detected. Preliminary negative report generated at 7 and 14 daysPerforming Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87102-Fungal culture, routine
87106-Yeast identification panel C (if appropriate)
87106-Yeast identification panel D (if appropriate)
87106-Id MALDI-TOF Mass Spec Yeast (if appropriate)
87107-Id MALDI-TOF Mass Spec Fungi (if appropriate)
87107-Fungal identification panel A (if appropriate)
87107-Fungal identification panel B (if appropriate)
87107-Yeast identification panel A (if appropriate)
87107-Yeast identification panel B (if appropriate)
87150-Identification rapid PCR Coccidioides (if appropriate)
87150 x 2-Identification Histoplasma/Blastomyces, PCR (if appropriate)
87153-D2 fungal sequencing identification (if appropriate)
87176-Tissue processing (if appropriate)