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HelpTest ID HPFLU Influenza Virus Type A and Type B, and Respiratory Syncytial Virus (RSV) RNA, Molecular Detection, PCR, Varies
Specimen Required
Preferred:
Specimen Type: Nasopharyngeal swab
Container/Tube: Sterile container with transport media
Specimen Volume: Entire specimen with a minimum of 1.5 mL (maximum 3 mL) of transport media.
Collection Instructions:
1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.
2. Swab must be placed into viral transport media (eg, M4-RT, M4 or M5), saline, or phosphate buffered saline (PBS). Media should not contain guanidine thiocyanate (GTC).
Acceptable:
Specimen Type: Oropharyngeal (throat) swab, nasal mid-turbinate, or nares/nasal swab
Supplies:
-Culturette (BBL Culture Swab) (T092)
-Mid-turbinate (MT) Swab (Copan FLOQSwab) (T864)
-Swab, Sterile Polyester (T507)
Container/Tube: Sterile container with transport media
Specimen Volume: Entire specimen with a minimum of 1.5 mL (maximum 3 mL) of transport media.
Preferred: BBL Culture Swab, COPAN Mid-turbinate Swab
Acceptable: Dacron-tipped swab with plastic handle
Collection Instructions: Swab must be placed into viral transport media (eg, M4-RT, M4, or M5), saline, or PBS. Media should not contain guanidine thiocyanate (GTC).
Specimen Type: Lower respiratory tract
Sources: Bronchoalveolar lavage (BAL), bronchial washings, endotracheal aspirate, sputum
Container/Tube: Sterile container
Specimen Volume: 0.6 mL
Additional Information: Do not aliquot into viral transport media.
Useful For
Simultaneous detection of influenza A virus, influenza B virus, and respiratory syncytial virus in upper or lower respiratory tract specimens from individuals with flu-like illnesses
Testing Algorithm
For information see Coronavirus Disease 2019 (COVID-19), Influenza, and Respiratory Syncytial Virus Testing Algorithm.
Method Name
Multiplex Real-Time Polymerase Chain Reaction (RT-PCR)
Reporting Name
Influenza A/B and RSV, PCR, VariesSpecimen Type
VariesSpecimen Minimum Volume
Swab in1.5 mL of media
Lower respiratory specimens: 0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Frozen (preferred) | 14 days |
| Refrigerated | 72 hours |
Reference Values
Undetected
Day(s) Performed
Monday through Sunday
Report Available
Same day/1 to 2 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87631
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HPFLU | Influenza A/B and RSV, PCR, Varies | 92143-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| HPFLS | Influenza A/B and RSV, Source | 31208-2 |
| 610412 | Influenza A virus PCR | In Process |
| 610413 | Influenza B virus PCR | In Process |
| 610414 | Respiratory Syncytial Virus, PCR | In Process |