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Test ID HSVPV Herpes Simplex Virus (HSV), Molecular Detection, PCR, Varies

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization


Ordering Guidance


If herpes simplex virus (HSV) is suspected in blood, order HSVPB / Herpes Simplex Virus (HSV), Molecular Detection, PCR, Blood.

 

If HSV is suspected in cerebrospinal fluid, order HSVC / Herpes Simplex Virus (HSV), Molecular Detection, PCR, Spinal Fluid.

 

If varicella-zoster virus is suspected, order VZVPV / Varicella-Zoster Virus, Molecular Detection, PCR, Varies.



Necessary Information


1. Specimen source is required.

2. Source information must include main anatomical site of collection.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Body fluid

Sources: Pleural, peritoneal, ascites, pericardial, amniotic, or ocular

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

 

Specimen Type: Swab

Sources: Genital, dermal, ocular, nasal, throat, or oral

Supplies:

-Culturette (BBL Culture Swab) (T092)

-BD E-Swab (T853)

-M4-RT (T605),

Container/Tube: Multimicrobe media (M4-RT, M4, M5, Bartels, or Jiangsu) and E-Swab or Culturette

Specimen Volume: Entire collection

Collection Instructions: Place swab back into multimicrobe media.

 

Specimen Type:Respiratory

Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate

Container/Tube: Sterile container

Specimen Volume: 1.5 mL

 

Specimen Type: Fresh tissue

Supplies: M4-RT (T605)

Container/Tube: Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4, M5, Bartels, or Jiangsu)

Specimen Volume: Entire collection

Collection Instructions: Submit only fresh tissue. Fixed tissue is not acceptable.

 

Specimen Type: Urine (newborns ≤35 days old)

Container/Tube: Sterile container

Specimen Volume: 0.5 mL


Reporting Name

Herpes Simplex Virus, PCR, Varies

Specimen Type

Varies

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Report Available

Same day/1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HSVPV Herpes Simplex Virus, PCR, Varies 94580-8

 

Result ID Test Result Name Result LOINC Value
HSVS Specimen Source 31208-2
618328 HSV 1, PCR 94581-6
618329 HSV 2, PCR 94582-4

Forms

If not ordering electronically, complete, print, and send Microbiology Test Request (T244) with the specimen.

Useful For

Aiding in the rapid diagnosis of herpes simplex virus (HSV) infections, including qualitative detection of HSV DNA in nonblood clinical specimens

 

This test should not be used to screen asymptomatic patients.

Day(s) Performed

Monday through Sunday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87529 x 2

87529 (if appropriate for government payers)

Specimen Minimum Volume

Amniotic or ocular fluid: 0.4 mL; Sterile body fluid (Pleural, peritoneal, ascites, pericardial): 0.5 mL; Respiratory Specimen: 1 mL; Swab, tissue, or urine: See Specimen Required

Reference Values

HERPES SIMPLEX VIRUS (HSV)-1

Negative

 

HERPES SIMPLEX VIRUS (HSV)-2

Negative