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Test ID JO1 Jo 1 Antibodies, IgG, Serum

Useful For

Evaluating patients with signs and symptoms compatible with a connective tissue disease, especially those patients with muscle pain and limb weakness, concomitant pulmonary signs and symptoms, Raynaud’s phenomenon, and arthritis

Testing Algorithm

See Connective Tissue Disease Cascade (CTDC) in Special Instructions.

Method Name

Multiplex Flow Immunoassay

Reporting Name

Jo 1 Ab, IgG, S

Specimen Type

Serum

Container/Tube: 

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.50 mL

Specimen Minimum Volume

0.35 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Clinical Information

Jo 1 (histidyl tRNA synthetase) is a member of the amino acyl-tRNA synthetase family of enzymes found in all nucleated cells. Jo 1 antibodies in patients with polymyositis bind to conformational epitopes of the enzyme protein and inhibit its catalytic activity in vitro.(1)

 

Jo 1 antibodies are a marker for the disease polymyositis, and occur most commonly in myositis patients who also have interstitial lung disease. The antibodies occur in up to 50% of patients with interstitial pulmonary fibrosis and symmetrical polyarthritis.(2)

 

See Connective Tissue Disease Cascade (CTDC) in Special Instructions and Optimized Laboratory Testing for Connective Tissue Diseases in Primary Care: The Mayo Connective Tissue Diseases Cascade in Publications.

Reference Values

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

Cautions

Testing for antibodies to Jo 1 is not useful in patients with a negative test for antinuclear antibodies.

 

A negative test for Jo 1 antibodies does not exclude the diagnosis of polymyositis or dermatomyositis.

Day(s) Performed

Monday through Saturday; 4 p.m., Sunday; 11a.m.

Report Available

1 day

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86235

NY State Approved

Yes