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Test ID KPNRP KPC (blaKPC) and NDM (blaNDM) in Gram-Negative Bacilli, Molecular Detection, PCR

Infectious

Useful For

Assessing pure isolates of gram-negative bacilli for mechanism of carbapenem resistance

Method Name

Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)

(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Reporting Name

KPC and NDM PCR

Specimen Type

Varies

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Klebsiella pneumoniae (KPC) or New Dehli metallo-beta-lactamase (NDM) DNA is unlikely.

 

Collection Container/Tube: Slant

Specimen Volume: Isolate

Collection Instructions:

1. Isolate infecting bacteria.

2. Organism must be in pure culture, actively growing. Do not submit mixed cultures.

3. Place specimen in a large infectious container (Supply T146) and label as an etiologic agent/infectious substance.

Additional Information:

1. Organism identification and specimen source are required.

2. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.

Forms: If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Clinical Information

Nonsusceptibility to carbapenems in gram-negative bacilli by means of the enzyme KPC (Klebsiella pneumoniae carbapenemase) or NDM (New Dehli metallo-beta-lactamase) is becoming more common. The genes blaKPC and blaNDM encode KPC and NDM enzyme production, respectively. In addition to KPC and NDM production, there are other mechanisms of resistance to carbapenems in gram-negative bacilli, including production of other carbapenemases, or plasmid-encoded AmpC, or extended beta-lactamase production combined with decreased membrane permeability. Detection of carbapenemases by the modified Hodge test may be subjective and is not rapid. Testing for the minimum inhibitory concentration (MIC) determines the level but not the mechanism of resistance. PCR is a sensitive, specific, and rapid means of detecting of a specific portion of the genes encoding KPC and NDM production.

Reference Values

Not applicable

Cautions

This assay should be used for testing of isolates of gram-negative bacilli. Request KNSRP / KPC (blaKPC) and NDM (blaNDM) Surveillance PCR, if testing directly from rectal or perirectal swabs is desired.

Day(s) Performed

Monday through Friday

Report Available

2 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87798 x 2

NY State Approved

Yes