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HelpTest ID LCMV Cytomegalovirus (CMV), Molecular Detection, PCR
Useful For
Rapid qualitative detection of cytomegalovirus DNA
Method Name
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe
Hybridization
(PCR is utilized pursuant to a license agreement with Roche
Molecular Systems, Inc.)
Reporting Name
Cytomegalovirus PCRSpecimen Type
VariesForms: If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
Microbiology Test Request Form (T244) (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf)
Neurology Test Request Form-General (T732) (http://www.mayomedicallaboratories.com/it-mmfiles/neurology-request-form.pdf)
Specimen source is required.
Submit only 1 of the following specimens:
Specimen Type: Fluid
Sources: Spinal, body, amniotic, ocular
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Respiratory
Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, tracheal aspirate
Container/Tube: Sterile container
Specimen Volume: 1.5 mL
Specimen Type: Genital
Sources: Cervix, vagina, urethra, anal/rectal, other genital sources
Container/Tube: BBL CultureSwab (T092)
Specimen Volume: Swab
Collection Instructions: Place swab back into swab cylinder.
Specimen Type: Miscellaneous
Sources: Dermal, eye, nasal, saliva, throat
Container/Tube: BBL CultureSwab (T092)
Specimen Volume: Swab
Collection Instructions: Place swab back into swab cylinder.
Specimen Type: Tissue
Sources: Brain, colon, kidney, liver, lung, etc.
Container/Tube: Sterile container with 1 to 2 mL of sterile saline or multi-microbe medium (M5) (T484)
Specimen Volume: Entire collection
Collection Instructions: Submit only fresh tissue.
Specimen Type: Urine
Container/Tube: Sterile container
Specimen Volume: 1 mL
Collection Instructions: Collect a random urine specimen.
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA)
Specimen Volume: 0.5 mL
Specimen Minimum Volume
Body/Ocular Fluid, Spinal Fluid, or Urine: 0.3 mL/Respiratory: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Refrigerated (preferred) | 7 days |
| Frozen | 7 days |
Clinical Information
Infection with cytomegalovirus (CMV) is a significant cause of morbidity and mortality in transplant recipients and other immunocompromised hosts. Specific neurologic syndromes associated with CMV infection include subacute radiculomyelopathy, peripheral neuropathy, and encephalitis.
CMV-associated central nervous system (CNS) disease occurs most commonly in immunocompromised patients. Histologic evidence of CMV infections in autopsy brain tissue was identified in 20% to 40% of AIDS patients. In 2 separate studies, CMV (DNA) was the most common herpesvirus (29/181, 16/49) detected from cerebrospinal fluid of patients with AIDS.
CNS infections with CMV can also occur in immunocompetent patients. CMV is a leading cause of congenital viral infections worldwide, and laboratory testing by real-time PCR is useful in the diagnosis of neonatal CMV disease.
Reference Values
Negative
Cautions
A negative result does not eliminate the possibility of cytomegalovirus (CMV) infection.
This assay is only to be used for patients with a clinical history and symptoms consistent with CMV infection, and must be interpreted in the context of the clinical picture. This test should not be used to screen asymptomatic patients.
Day(s) Performed
Monday through Saturday; varies
Report Available
1 day/same dayPerforming Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
87496