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HelpTest ID LSDX Lysergic Acid Diethylamide (LSD) Confirmation, Chain of Custody, Urine
Useful For
Confirming use of lysergic acid diethylamide
Chain of custody is required whenever the results of testing could be used in a court of law. Its purpose is to protect the rights of the individual contributing the specimen by demonstrating that it was under the control of personnel involved with testing the specimen at all times; this control implies that the opportunity for specimen tampering would be limited.
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| COCH | Chain of Custody Processing | Yes | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ADLTX | Adulterants Survey, CoC, U | Yes | No |
Testing Algorithm
Adulterants testing will be performed on all chain of custody urine samples as per regulatory requirements.
Method Name
Immunoassay Followed by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Confirmation with Quantitation
Reporting Name
LSD Confirmation, CoC, USpecimen Type
UrineContainer/Tube: Chain-of-Custody Kit (T282) containing the specimen containers, seals, and documentation required.
Specimen Volume: 20 mL
Collection Instructions: Collect specimen in the container provided, seal, and submit with the associated documentation to satisfy the legal requirements for chain-of-custody testing.
Additional Information
1. No specimen substitutions.
2. No STATS are accepted for this procedure.
3. Submitting <20 mL will compromise our ability to perform all necessary testing.
Forms: Chain-of-Custody Request Form is included in the Chain-of-Custody Kit (T282). A copy of this form is also available at http://www.mayomedicallaboratories.com/it-mmfiles/chain-of-custody-request-form.pdf.
Specimen Minimum Volume
5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Urine | Refrigerated (preferred) | 14 days |
| Frozen | 14 days | |
| Ambient | 72 hours |
Clinical Information
Lysergic acid diethylamide (LSD) is an extremely potent psychedelic ergot alkaloid derived from the fungus, Claviceps purpurea.(1) The drug LSD binds to serotonin receptors in the central nervous system and acts as a serotonin agonist.
The clearance half-life of LSD averages 3 to 4 hours. It takes 5 to 7 half-lives to clear 98% of a drug dose. Therefore, the presence of LSD greater than the lower limit of quantification (LOQ; cutoff concentration) indicates exposure to LSD within 1 day.(2)
LSD is metabolized to 2-oxo-3-hydroxy-LSD, which is present at concentrations 16 to 43 times higher than LSD, and is likely to be present in urine for up to 4 days after last exposure.(3,4)
Chain of custody is a record of the disposition of a specimen to document who collected it, who handled it, and who performed the analysis. When a specimen is submitted in this manner, analysis will be performed in such a way that it will withstand regular court scrutiny.
Reference Values
Negative
Cutoff concentrations:
IMMUNOASSAY SCREEN
<0.5 ng/mL
LSD BY LC-MS/MS
<0.5 ng/mL
2-OXO-3-HYDROXY-LSD BY LC-MS/MS
<5.0 ng/mL
Cautions
The liquid chromatography-tandem mass spectrometry (LC-MS/MS) test is not subject to any known interference.
Positive immunoassay screening results should be considered presumptive due to documented cross-reactivities with the lysergic acid diethylamide reagent antibody.
Day(s) Performed
Wednesday
Report Available
2 daysPerforming Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
80323
G0480 (if appropriate)