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HelpTest ID MGRP Mycoplasma genitalium, Molecular Detection, PCR
Useful For
Rapid, sensitive, and specific identification of Mycoplasma genitalium from genitourinary and reproductive sources
Method Name
Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and
Fluorescent Resonance Energy Transfer (FRET)
(PCR is utilized pursuant to a license agreement with Roche
Molecular Systems, Inc.)
Reporting Name
Mycoplasma genitalium PCRSpecimen Type
VariesThe high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasma genitalium DNA is not likely.
Forms: If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).
Specimen source is required.
Submit only 1 of the following specimens:
Specimen Type: Swab
Sources: Cervix, urethra, urogenital, vaginal
Container/Tube:
Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)
Acceptable: Swab in transport media: M4, M5 (Supply T484), universal transport medium, or ESwab
Specimen Volume: One Swab
Collection Instructions:
1. Vaginal: Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.
2. Urethra or Cervical: Collect specimen by inserting swab 1 to 3 cm and rotating 360 degrees.
3. Place swab back into swab cylinder.
Specimen Type: Fluid
Sources: Amniotic, pelvic, prostatic secretion, reproductive drainage, semen
Container/Tube:
Preferred: Sterile container
Acceptable: Container with 3 mL of transport media: M4, M5 (Supply T484), or universal transport media
Specimen Volume: 1-2 mL
Specimen Type: Urine, kidney/bladder stone, ureter
Container/Tube: Sterile container
Specimen Volume: 10 mL or entire specimen
Specimen Type: Tissue
Sources: Placenta, products of conception, genitourinary
Container/Tube: Sterile container
Specimen Volume: 5 mm
Collection Instructions: Submit only fresh tissue.
Specimen Minimum Volume
Pelvic Fluid, Amniotic Fluid, Prostatic Secretions, Semen, Reproductive Drainage or Fluid: 1 mL; Urine: 2 mL; Swab: 1 swab
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Refrigerated (preferred) | 7 days |
| Frozen | 7 days |
Clinical Information
Mycoplasma genitalium causes acute and chronic nongonococcal urethritis, cervicitis, and pelvic inflammatory disease. Culture isolation is technically challenging; PCR is the diagnostic test of choice.
Reference Values
Not applicable
Cautions
Interfering substances may affect the accuracy of this assay; results should always be interpreted in conjunction with clinical and epidemiological findings.
This test does not detect other mycoplasmas or ureaplasmas.
Day(s) Performed
Monday through Friday
Report Available
3 daysPerforming Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
87798