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Test ID PK Pyruvate Kinase, Erythrocytes

Useful For

Work-up of cases of nonspherocytic hemolytic anemia

 

Investigating families with pyruvate kinase deficiency to determine inheritance pattern and for genetic counseling

Method Name

Kinetic Spectrophotometry (KS)

Reporting Name

Pyruvate Kinase, RBC

Specimen Type

Whole Blood ACD-B

Container/Tube:

Preferred: Yellow top (ACD solution B)

Acceptable: EDTA

Specimen Volume: 6 mL

Collection Instructions: Do not transfer blood to other containers.

Forms: If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf)

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood ACD-B Refrigerated 20 days

Clinical Information

Deficiencies of most of the enzymes of the Embden-Meyerhof (glycolytic) pathway, including pyruvate kinase (PK), have been reported. PK deficiency, although rare, is the erythrocyte enzyme deficiency most frequently found to be a cause of congenital nonspherocytic hemolytic anemia. It is an autosomal recessive disorder. Thus, the parents of affected patients are heterozygotes. Patients usually present during early childhood with anemia, icterus, and splenomegaly. Hemolytic disease of the newborn is common in persons with PK deficiency.

Reference Values

≥12 months: 6.7-14.3 U/g Hb

Reference values have not been established for patients who are <12 months of age.

Cautions

Because leukocytes also contain pyruvate kinase (PK) that is not diminished in hereditary erythrocytic PK deficiency, freeing the blood of white blood cells is always critical to this test.

Day(s) Performed

Monday through Saturday

Report Available

1 day

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

84220

NY State Approved

Yes