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HelpTest ID SEQB Sequential Maternal Screening, Part 2, Serum
Ordering Guidance
Do not order this test unless test SEQA / Sequential Maternal Screening, Part 1, Serum has already been ordered.
If a standalone second-trimester screen is desired, order QUAD1 / Quad Screen (Second Trimester) Maternal, Serum.
If a stand-alone neural tube defect risk assessment is desired, order MAFP1 / Alpha-Fetoprotein (AFP), Single Marker Screen, Maternal, Serum.
Additional Testing Requirements
Sequential maternal screening is a 2-step test that includes a first-trimester sample (SEQA / Sequential Maternal Screening, Part 1, Serum) and a second-trimester sample (SEQB / Sequential Maternal Screening, Part 2, Serum).
Necessary Information
Collection date is required.
Specimen Required
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Collect blood between 15 weeks, 0 days and 22 weeks, 6 days. Do not collect blood after performing amniocentesis, as that may lead to an artificially increased serum alpha-fetoprotein level and unreliable results.
2. Centrifuge and aliquot serum into a plastic vial within 2 hours of collection.
Useful For
Prenatal screening for Down syndrome, neural tube defects, and trisomy 18
Identifying abnormal levels of alpha-fetoprotein in the second trimester
Special Instructions
Method Name
Immunoenzymatic Assay
Reporting Name
Sequential Maternal Screen, Part 2Specimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 7 days |
| Frozen | 90 days | |
| Ambient | 7 days |
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Friday
Report Available
4 to 6 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| SEQB | Sequential Maternal Screen, Part 2 | 48800-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 29476 | Recalculated Maternal Serum Screen | 43995-0 |
| 601813 | Results Summary | 49092-0 |
| 29500 | Down Syndrome Screen Risk Estimate | 43995-0 |
| 29501 | Down Syndrome Maternal Age Risk | 49090-4 |
| 29502 | Trisomy 18 Screen Risk Estimate | 43994-3 |
| 602040 | Neural Tube Defect Risk Estimate | 49091-2 |
| 29491 | NT | 49035-9 |
| 601814 | NT MoM | 49035-9 |
| 29492 | NT Twin | 49035-9 |
| 601815 | NT Twin MoM | 49035-9 |
| 29887 | PAPP-A | 48407-1 |
| 601816 | PAPP-A MoM | 76348-2 |
| 29494 | AFP | 20450-3 |
| 601817 | AFP MoM | 23811-3 |
| 602805 | AFP MoM (14,0-14,6) | 23811-3 |
| 29495 | uE3 | 20466-9 |
| 601818 | uE3 MoM | 21264-7 |
| 29496 | hCG, Total | 83086-9 |
| 601819 | hCG, Total MoM | 23841-0 |
| 29497 | Inhibin | 35738-4 |
| 601820 | Inhibin MoM | 36904-1 |
| 29503 | Interpretation | 49092-0 |
| 29505 | Recommended Follow Up | 80615-8 |
| 29504 | Additional Comments | 48767-8 |
| 29477 | Specimen Collection Date 1 | 33882-2 |
| 29493 | Specimen Collection Date 2 | 33882-2 |
| 29478 | Maternal Date of Birth | 21112-8 |
| 29892 | Calculated age at EDD | 43993-5 |
| 29479 | Maternal Weight | 29463-7 |
| 29481 | Insulin Dependent Diabetes | 33248-6 |
| 29482 | Patient Race | 32624-9 |
| 601811 | Current cigarette smoking status | 72166-2 |
| 29485 | Scan Date | 34970-4 |
| 29488 | CRL | 11957-8 |
| 29489 | CRL Twin | 11957-8 |
| 29893 | GA on Collection by U/S Scan 1 | 11888-5 |
| 29894 | GA on Collection by U/S Scan 2 | 11888-5 |
| 29886 | Number of Fetuses | 11878-6 |
| 29490 | Chorions | 92568-5 |
| 29888 | IVF | 47224-1 |
| 601808 | Prev Down (T21) / Trisomy Pregnancy | 53826-4 |
| 601809 | Prev Pregnancy w/ Neural Tube Defects | 53827-2 |
| 601807 | Initial or repeat testing | 89231-5 |
| 601810 | Patient or father of baby has a NTD | 53827-2 |
| 601803 | Sonographer Name | 49088-8 |
| 602041 | Sonographer Code | No LOINC Needed |
| 601812 | Sonographer Reviewer ID | 49089-6 |
| 601804 | Physician Phone Number | 68340-9 |
| 29506 | General Test Information | 62364-5 |
CPT Code Information
81511
82105 (if appropriate)
82677 (if appropriate)
84702 (if appropriate)
86336 (if appropriate)
Testing Algorithm
For more information see Sequential Maternal Serum Screening Testing Algorithm.