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HelpTest ID SFMON Hemoglobin S and Hemoglobin F Quantitation for Therapeutic Monitoring, Blood
Useful For
Monitoring patients with sickling disorders who have received hydroxyurea or transfusion therapy
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| SDEX | Hemoglobin S, Scrn, B | Yes | No |
Testing Algorithm
If hemoglobin S is detected, hemoglobin S screen will be performed, when appropriate, at an additional charge.
Method Name
Cation Exchange/High-Performance Liquid Chromatography (HPLC)
Reporting Name
Hb S/F Therapeutic Monitoring, BSpecimen Type
Whole bloodContainer/Tube:
Preferred: Lavender top (EDTA)
Acceptable: ACD, heparin
Specimen Volume: 6 mL
Collection Instructions: Do not transfer blood to other containers.
Additional Information: Patient's age is required.
Forms: If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf)
Specimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole blood | Refrigerated | 7 days |
Clinical Information
The treatment of red blood cell sickling disorders may involve many therapeutic modalities. Two of the most important and beneficial are treatment with hydroxyurea and chronic transfusion therapy. Hydroxyurea causes elevation of hemoglobin F (Hb F) levels, and transfusion serves to lower the percentage of hemoglobin S (Hb S). Both of these therapeutic modalities act to lessen the number and severity of sickling crises. Thus, periodic monitoring of Hb F and Hb S levels are needed to guide further therapy.
Reference Values
HEMOGLOBIN F
1-30 days: 22.8-92.0%
1-2 months: 7.6-89.8%
3-5 months: 1.6-42.2%
6-8 months: 0.0-16.7%
9-12 months: 0.0-10.5%
13-17 months: 0.0-7.9%
18-23 months: 0.0-6.3%
≥24 months: 0.0-0.9%
HEMOGLOBIN S
All ages: 0.0%
Cautions
This test is not intended for diagnostic purposes; thus, it is assumed the patient's diagnosis is established. If the patient has never been studied, hemoglobin electrophoresis is necessary (see HBELC / Hemoglobin Electrophoresis Cascade, Blood).
Day(s) Performed
Monday through Saturday; 7 a.m. and 1 p.m.
Report Available
Same day/1 dayPerforming Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
83021-Quantitation by HPLC
85660-Sickling of RBC, reduction (if appropriate)