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HelpTest ID TCAPF T- and B-Cell Quantitation and Lymphocyte Proliferation to Antigens Panel
Useful For
Assessing antigen-specific T-cell responses and quantitating lymphocyte subsets in a single orderable test.
This panel is most helpful when evaluating patients with immunodeficiencies, where quantitative decreases in T cells can lead to reduced functional antigen responses.
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| TBBS | T- and B-Cell QN by Flow Cytometry | Yes | Yes |
| LPAGF | Lymphocyte Proliferation, Antigens | Yes | Yes |
Testing Algorithm
When multiple specimen types are required to perform a panel of tests, the laboratory will perform the tests for which the appropriate specimen type was received and the laboratory will cancel those for which the appropriate specimen was not received. Be advised that this may change the degree of interpretation received with the report.
Method Name
Flow Cytometry
Reporting Name
T-Cell with Antigen PanelSpecimen Type
WB Sodium HeparinWhole Blood EDTA
Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. It is recommended that specimens arrive within 24 hours of draw. Samples arriving on the weekend may be canceled. Draw and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Mailer-Critical Specimens Only (Supply T668).
Date and time of draw and ordering physician's name and phone number are required.
For serial monitoring, we recommend that specimen draws be performed at the same time of day.
Forms: If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf)
Two separate specimens are required.
Specimen Type: EDTA whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions: Send specimen in original tube. Do not aliquot.
Specimen Type: Sodium heparin whole blood
Container/Tube: Green top (sodium heparin)
Specimen Volume:
<3 months: 1 mL
3-24 months: 3 mL
25 months-18 years: 5 mL
>18 years: 20 mL
Collection Instructions: Send specimen in original tube. Do not aliquot.
Specimen Minimum Volume
EDTA whole blood: 1 mL/Sodium heparin whole blood: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| WB Sodium Heparin | Ambient | 48 hours |
| Whole Blood EDTA | Ambient | 52 hours |
Clinical Information
See individual test IDs.
Reference Values
The appropriate age-related reference values will be provided on the report.
Cautions
See individual test IDs.
Day(s) Performed
T- and B-Cell quantitation by flow cytometry: Monday through Sunday
Lymphocyte proliferation to antigens: Monday through Friday. Specimens are required to be received in the lab weekdays and by 4 p.m. on Friday. No weekend processing.
Report Available
11 daysPerforming Laboratory
Mayo Medical Laboratories in Rochester
Test Classification
See Individual Test IDsCPT Code Information
T- and B-Cell Quantitation by Flow Cytometry
86355-B cells, total count
86357-Natural killer (NK) cells, total count
86359-T cells, total count
86360-Absolute CD4/CD8 count with ratio
Lymphocyte Proliferation to Antigens, Blood
86353