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Test ID TLPMF T-Cell Lymphoma, Specified FISH, Varies


Ordering Guidance


This test is intended for instances when limited T-cell lymphoma fluorescence in situ hybridization (FISH) probes are needed. The FISH probes to be analyzed must be specified on the request, otherwise test processing may be delayed in order to determine intended analysis. If specific probes are not included with this test request, the test may be canceled and automatically reordered by the laboratory as TLPDF / T-Cell Lymphoma, Diagnostic FISH, Varies.

 

If the entire T-cell lymphoma panel is preferred, order TLPDF / T-Cell Lymphoma, Diagnostic FISH, Varies.

 

For patients with T-cell acute lymphoblastic leukemia/lymphoma (T-ALL/LBL), order either TALAF / T-Cell Acute Lymphoblastic Leukemia/Lymphoma (ALL), FISH, Adult, Varies. or TALPF / T-Cell Acute Lymphoblastic Leukemia/Lymphoma (ALL), FISH, Pediatric, Varies depending on the age of the patient.

 

For testing paraffin-embedded tissue samples from patients with T-lymphoblastic lymphoma (T-BLB), see TLBLF / T-Lymphoblastic Leukemia/Lymphoma, FISH, Tissue.



Shipping Instructions


Advise Express Mail or equivalent if not on courier service.



Necessary Information


1. A list of probes requested for analysis is required. Probes available for this test are listed in the Testing Algorithm section.

2. A reason for testing and a flow cytometry and/or a bone marrow pathology report should be sent with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed. If this information is not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.



Specimen Required


Submit only 1 of the following specimens:

 

Preferred

Specimen Type: Bone marrow

Container/Tube:

Preferred: Yellow top (ACD)

Acceptable: Green top (heparin) or lavender top (EDTA)

Specimen Volume: 2-3 mL

Collection Instructions:

1. It is preferable to send the first aspirate from the bone marrow collection.

2. Invert several times to mix bone marrow.

 

Acceptable

Specimen Type: Blood

Container/Tube:

Preferred: Yellow top (ACD)

Acceptable: Green top (heparin) or lavender top (EDTA)

Specimen Volume: 6 mL

Collection Instructions: Invert several times to mix blood.


Forms

If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Secondary ID

614346

Useful For

Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with various T-cell lymphomas using client specified probes

 

Tracking known chromosome abnormalities and response to therapy in patients with T-cell lymphoma

 

Evaluating specimens in which standard cytogenetic analysis is unsuccessful

Testing Algorithm

This test includes a charge for application of the first probe set (2 fluorescence in situ hybridization [FISH] probes) and professional interpretation of results. Analysis charges will be incurred based on the number of cells analyzed per probe set.

 

When specified, any of the following probes will be performed:

14q32.1 rearrangement, TCL1A break-apart

14q11.2 rearrangement, TRAD break-apart

i(7q), D7Z1/D7S486

+8, D8Z2/MYC

 

For more information see Bone Marrow Staging for Known or Suspected Malignant Lymphoma Algorithm.

Method Name

Fluorescence In Situ Hybridization (FISH)

Reporting Name

T-cell Lymphoma B/BM, Spec FISH

Specimen Type

Varies

Specimen Minimum Volume

Blood: 2 mL
Bone Marrow: 1 mL

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Clinical Information

T-cell neoplasms are relatively uncommon, accounting for approximately 12% of all non-Hodgkin lymphomas. There are several subtypes of T-cell neoplasms: T-lymphoblastic leukemia , T-cell prolymphocytic leukemia , T-cell large granular lymphocytic leukemia , anaplastic large cell lymphoma , peripheral T-cell lymphoma, and various other cutaneous, nodal, and extranodal lymphoma subtypes.

 

There are a few common chromosome abnormalities associated with specific T-cell lymphoma subtypes evaluated by this FISH test as seen in the following table.

 

Table. Common Chromosome Abnormalities in T-Cell Lymphomas

Lymphoma subtype

Chromosome abnormality

Fluorescence in situ hybridization probe

T-cell prolymphocytic leukemia/lymphoma (T-PLL)

inv(14)(q11q32) and t(14;14)(q11;q32)

5'/3'TCL1A

     Reflex: 14q11.2 rearrangement

5'/3'TRAD

Hepatosplenic T-cell lymphoma

Isochromosome 7q

D7S486/D7Z1

Trisomy 8

D8Z2/MYC

 

These probes have diagnostic relevance and can also be used to track response to therapy.

Reference Values

An interpretive report will be provided.

Cautions

This test is not approved by the U.S. Food and Drug Administration, and it is best used as an adjunct to existing clinical and pathologic information.

 

Bone marrow is the preferred sample type for this fluorescence in situ hybridization test. If bone marrow is not available, a blood specimen may be used if there are neoplastic cells in the blood specimen (as verified by a hematopathologist).

Day(s) Performed

Monday through Friday

Report Available

7 to 10 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88271x2, 88275 x1, 88291x1- FISH Probe, Analysis, Interpretation; 1 probe set

88271x2, 88275 x1 - FISH Probe, Analysis; each additional probe set (if appropriate)

NY State Approved

Yes