Test ID TTF40 TTF41 (SPT24) + p40 Immunostain, Technical Component Only

Shipping Instructions

Attach the green pathology address label and the pink Immunostains Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Specimen Type: Tissue

Supplies: Immunostain Technical Only Envelope (T693)

Container/Tube: Immunostains Technical Only Envelope (T693)

Preferred: 2 unstained positively charged glass slide (25 x 75 x 1mm) per test ordered; sections 4-microns thick

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Forms

If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Secondary ID

602647

Useful For

Thyroid transcription factor 1 aids in the classification of carcinomas of unknown origin

p40 aids in the classification of carcinomas and lymphomas

Reflex Tests

Test ID	Reporting Name	Available Separately	Always Performed
IHMTO	IHC Multiplex, Tech Only	No	No

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Method Name

Immunohistochemistry

Reporting Name

TTF1 (SPT24) + p40 IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type	Temperature	Time
TECHONLY	Ambient (preferred)
	Refrigerated

Clinical Information

Thyroid transcription factor 1 (TTF1) is a nuclear protein (detected by the chromogen 3,3'-diaminobenzidine: DAB) expressed in thyroid follicular cells, type II pneumocytes, and a subset of bronchial cells. The p40 antibody recognizes the deltaNp63 isoform of p63 (detected by the chromogen fast red). The predominant localization of p40 is in the basal layer of the stratified squamous and transitional epithelia. Given the relative specificity of TTF1 for cells of thyroid or lung origin, TTF1 is often included in a panel to identify the primary site for carcinomas of unknown origin. The p40 antibody may help to distinguish squamous cell carcinomas from other non-small cell carcinomas.

Reference Values

This is not an orderable test. Order PATHC / Pathology Consultation. The consultant will determine the need for special stains.

Cautions

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88344-TC

NY State Approved

Yes

Mayo Clinic Laboratories Extended Catalog

Search

Browse by Name