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Test ID VITB2 Riboflavin (Vitamin B2), Plasma

Useful For

Evaluation of persons who present the signs of ariboflavinosis

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Riboflavin (Vitamin B2), P

Specimen Type

Plasma Heparin

Collection Container/Tube: Green top (heparin)

Submission Container/Tube: Amber vial (Supply T192)

Specimen Volume: 0.50 mL

Collection Instructions: 

1. Fasting-overnight (12-14 hours) (infants-draw prior to next feeding).

2. Send specimen in amber vial to protect from light.

Specimen Minimum Volume

0.25 mL plasma

Specimen Stability Information

Specimen Type Temperature Time
Plasma Heparin Frozen (preferred) 14 days
  Refrigerated  7 days

Clinical Information

There are 3 principle vitamin B2-active flavins found in nature-riboflavin, riboflavin 5-phosphate (flavin mononucleotide [FMN]), and riboflavin-5'-adenosyl-diphosphate (flavin adenosine dinucleotide [FAD]). In biological tissues, FMN and FAD serve as prosthetic units for a large variety of flavoproteins, which are hydrogen carriers in oxidation-reduction processes.

 

Dietary deficiency of riboflavin (ariboflavinosis) is characterized by sore throat, cheilosis (lesions on the lips), angular stomatitis (lesions on the angles of the mouth), glossitis (fissured and magenta-colored tongue), corneal vascularization, dyssebacia (red, scaly, greasy patches on the nose, eyelids, scrotum, and labia), and normocytic, normochromic anemia. Severe riboflavin deficiency may affect the conversion of vitamin B6 to its coenzyme, as well as conversion of tryptophan to niacin.

 

Riboflavin has a low level of toxicity and no case of riboflavin toxicity in humans has been reported. The limited absorptivity of riboflavin and its ready excretion in the urine normally preclude a health problem due to increased intake of riboflavin.

Reference Values

Normal: 1-19 mcg/L

Cautions

Testing of nonfasting specimens or the use of dietary vitamin B2 supplementation can result in elevated plasma vitamin B2 concentrations.

Day(s) Performed

Tuesday, Thursday

Report Available

2 days (not reported on Saturday or Sunday)

Performing Laboratory

Mayo Medical Laboratories in New England

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

84252

NY State Approved

Conditional